The effect of regulator proteins on the IFN-γ/TLR9 synergistic signal transduction

Baljinnyam T; Khulan O; Erkhembayar Sh; Baasansuren E; Jawkhlan B; Batkhishig; Enkhsaikhan L; Galindew B; Tsewelmaa N; Baigalmaa B; Hongorzul B; Sodnomtsogt L; Nyambayar D; Batbaatar G; Monhbat B; Munkhtuwshin N; Bilegtsaikhan Ts

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The effect of regulator proteins on the IFN-γ/TLR9 synergistic signal transduction

VernacularTitle:IFN-γ/TLR9-ийн харилцан дэмжих үйлчлэлд зохицуулагч молекулын нөлөө
Author: Baljinnyam T ^{1
,

2} ; Khulan O ² ; Erkhembayar Sh ³ ; Baasansuren E ² ; Jawkhlan B ² ; Batkhishig ² ; Enkhsaikhan L ² ; Galindew B ² ; Tsewelmaa N ² ; Baigalmaa B ² ; Hongorzul B ² ; Sodnomtsogt L ² ; Nyambayar D ² ; Batbaatar G ² ; Monhbat B ² ; Munkhtuwshin N ¹ ; Bilegtsaikhan Ts ^{1
,

2}
Author Information

1. Core Laboratory, Science Technology Center, MNUMS
2. Department of Microbiology and Immunology, SBS, MNUMS
3. Central Scientific Research Laboratory, Institute of Medical Sciences, Mongolia
Publication Type:Journal Article
From: Health Laboratory 2018;8(1):8-13
CountryMongolia
Language:Mongolian
Abstract: Introduction:When human body encounters external pathogens primary/innate immunity cells are activated by recognizing them and secondary/adaptive immunity is activated consecutively. Immune cell surface receptors, called Toll-like receptors (TLRs) recognize and bind pathogens. In our previous study, we revealed that there is a synergistic action between TLR9 and IFN-γ signaling in the endothelial cells.
Purpose:To determine the role of negative and positive regulatory proteins on the IFN-γ/TLR9 synergistic signaling pathway
Materials and Methods:This study was held in the Core Laboratory, Science Technology Center, Mongolian National University of Medical Sciences (MNUMS). In this study, murine endothelial cell (END-D) culture was used. The negative and positive regulator protein expression was detected by Western blotting.
Result:Result of immunoblotting assay indicated that CpG DNA enhanced IFN-γ positive regulator protein p38 phosphorylation in the endothelial cells. Treatment by TLR9 ligand CpG DNA and IFN-γ increased p38 activation in 0.5 hour and 1 hour. CpG DNA inhibited IFN-γ negative regulator SOCS1 protein expression in 4 hr and 8 hr. Therefore, TLR9 ligand CpG DNA increased IFN-γ signal transduction in the endothelial cell line.
Conclusion:TLR9 ligand CpG DNA has decreased IFN-γ negative regulator protein SOCS1 expression. CpG DNA has increased IFN-γ positive regulator protein p38 phosphorylation.
Full text:HL-2018-8(2)-8-13.pdf