Dysfunction of endothelial progentior cells in the angiographically defined coronary atherosclerosis
- VernacularTitle:Титэм судасны хатуурлын үед эндотелийн прогенитор эсийн үйл ажиллагааг судалсан нь
- Author:
Sumiya Tserendavaa
1
;
Odkhuu Enkhtaivan
1
;
Tsogtsaikhan Sandag
1
;
Chinzorig Ganbaatar
1
;
Davaakhuu Sengebaljir
2
;
Munkhzol Malchinkhuu
1
Author Information
1. School of Biomedicine, Mongolian National University of Medical Sciences
2. Ojinmed Hospital
- Publication Type:Journal Article
- Keywords:
Coronary atherosclerosis;
acute myocardial infarction;
stable angina pectoris;
endothelial progenitor cell;
Colony forming units;
endothelial nitric oxide synthetase
- From:Mongolian Medical Sciences
2018;183(1):3-8
- CountryMongolia
- Language:Mongolian
-
Abstract:
Introduction:Endothelial progenitor cells (EPC) have a role in the maintenance and promotion of vascular repair and are negatively correlated with coronary atherosclerosis.
Goal:To culture of EPC-CFUs during coronary atherosclerosis, evaluate endothelial nitric oxide synthetase (eNOS) enzyme levels in the culture.
Materials and Methods:The 10 ml blood was drawn from the peripheral vein of 12 man patients that stable angina 4, acute myocardial infarction (AMI) 4 and healthy people 4. Peripheral blood mononuclear cells were isolated by Ficoll density-gradient centrifugation and EPC-CFUs was assayed after two platings and a 6 day culture on fibronectin coated, 72 well plates, as described. eNOS enzyme titers were determined by ELISA according to the protocol in the cells culture.
Results:The people were 52±2.12 years. The number of EPC-CFUs increases with accordance of patients with stable angina, AMI, healthy people with the statistical significance (F=17.3, p<0.001): stable angina (2.6±0.47 colony/well), AMI (6.7±0.81 colony/well), healthy people (10.5±1.34 colony/well). Furthermore, ANOVA test of eNOS enzyme levels in patients with stable angina (5.2±0.61 pg/ml), AMI (8.7±1.49 pg/ml) and healthy people (13.7±2.48 pg/ml). The significant difference (F=6.2, p=0.003) was observed among the three groups. The number of EPC-CFUs had direct significantly correlation (r=0.621, p<0.001) with the eNOS enzyme levels of this culture.
Conclusion:Number of EPC-CFUs and eNOS enzyme levels decrease at patient with stable angina, indicate more than endothelial dysfunction.
Ethical approval:The ethics committee of Mongolian National University of Medical Sciences (ID: 6/3/201506, approved on Jan 01, 2015)
- Full text:2018-183(1)-3-8.pdf
