Primer probes for targeted identification and detection of Bifidobacterium animalis and their application
10.11665/j.issn.1000-5048.20220912001
- VernacularTitle:靶向鉴别、检测动物双歧杆菌的引物探针及应用
- Author:
Lixia DU
1
;
Dian LI
;
Huirong ZHUO
;
Mengnan SHI
;
Yanning CHEN
;
Lanfang LI
;
Shaoyang HOU
Author Information
1. 菏泽学院药学院
- Publication Type:Journal Article
- Keywords:
Bifidobacterium animalis;
ERIC-PCR;
bacterial identification;
primer probe
- From:
Journal of China Pharmaceutical University
2023;54(2):255-262
- CountryChina
- Language:Chinese
-
Abstract:
In this study, in order to overcome the shortcomings of the current methods used to identify Bifidobacterium animalis, such as long time, complicated operation and low adaptability of experimental environment, specific primer probes were designed based on ERIC-PCR technology to identify and detect B.animalis.Based on the genomic DNA of B.animalis HP-B1124, the ERIC-PCR reaction conditions of B.animalis HP-B1124 were optimized, and the ERIC-PCR fragments were obtained one by one and sequenced.Two pairs of specific primer probes were designed.The accuracy, specificity, limitation and universality of the two pairs of primer probes were evaluated, and the two pairs of specific primer probes were used for testing the products containing B.animalis in the commercially published formula.The two pairs of specific primer probes designed in this study could be used for identified strains of B.animalis more simply, quickly and targeted.This method has optimized the current relatively traditional methods of pure culture and plate counting identification of B.animalis, and has solved the high requirements of SNP genotyping technology and real-time fluorescence quantitative PCR method for experimental equipment and reagents in the identification of B.animalis to a certain extent.It has the characteristics of low cost, high specificity and earn a broad market development prospect.
