Effects and mechanisms of SNP-9 on Parkinson''s disease cell model induced by rotenone

VernacularTitle:SNP-9对鱼藤酮所致帕金森病细胞模型的作用及机制研究
Author: Zhihuan HUANG ¹ ; Xiao LIANG ; Xiangdong GAO ; Song CHEN
Author Information

1. 中国药科大学生命科学与技术学院江苏省生物药物成药性研究重点实验室
Publication Type:Journal Article
Keywords: Parkinson''s disease; SNP-9; rotenone; apoptosis
From: Journal of China Pharmaceutical University 2023;54(2):238-244
CountryChina
Language:Chinese
Abstract: In this article, the effects and mechanisms of SNP-9 on Parkinson''s disease (PD) cell model were investigated.SH-SY5Y cells were treated with rotenone to establish PD cell model; the effects of rotenone and SNP-9 on cell viability were detected by MTT assay; Hoechst/PI double staining assay was used to detect the effects of rotenone and SNP-9 on cell apoptosis; DCFH-DA probe was used to detect the effects of rotenone and SNP-9 on cellular reactive oxygen species (ROS) levels; and Western blot was used to detect the effects of rotenone and SNP-9 on protein levels of tyrosine hydroxylase (TH), α-synuclein (α-syn), Bcl-2 and Bax.The results showed that SNP-9 could alleviate abnormalities in cell viability, levels of TH and α-syn, apoptosis, ROS and apoptotic relative protein Bax/Bcl-2 induced by rotenone.Our findings suggest that SNP-9 may alleviate rotenone-induced injury in neuronal cells by regulating cell apoptosis related pathway.