Evaluation of beak and feather disease virus, avian polyomavirus and avian papillomavirus of captives psittacine birds in Seri Kembangan, Selangor, Malaysia

Muhammad Faiz Mohd Padzil; Nur Syafiqah Ab Halim; Nurulhuda Najihah; Aminuddin Baki Nurul Najian; Jalila Abu; Nurulfiza Mat Isa; Han Yih Lau; Abdul Razak Mariatulqabtiah

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Evaluation of beak and feather disease virus, avian polyomavirus and avian papillomavirus of captives psittacine birds in Seri Kembangan, Selangor, Malaysia

Author: Muhammad Faiz Mohd Padzil ¹ ; Nur Syafiqah Ab Halim ² ; Nurulhuda Najihah ¹ ; Aminuddin Baki Nurul Najian ² ; Jalila Abu ³ ; Nurulfiza Mat Isa ^{4
,

5} ; Han Yih Lau ⁶ ; Abdul Razak Mariatulqabtiah ^{4
,

5}
Author Information

1. Laboratory of Vaccines and Biomolecules, Institute of Bioscience, Universiti Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia.
2. Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia.
3. Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia.
4. Laboratory of Vaccines and Biomolecules, Institute of Bioscience, Universiti Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia. &
5. Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia.
6. Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), Persiaran MARDI-UPM, 43400 Serdang, Selangor, Malaysia.
Publication Type:Other Types
Keywords: Avian viruses; Beak and feather disease virus (BFDV); Avian polyomavirus; Avian papillomavirus; Psittacine
MeSH: Virus Diseases; Birds
From:Malaysian Journal of Microbiology 2021;17(3):338-344
CountryMalaysia
Language:English
Abstract: Aims:Psittacine birds such as parrots, macaws, cockatoos, lovebirds and parakeets, are widely reared as household pets or at aviary due to their attractive features. However, the status of virus-causing diseases of psittacine species in Malaysia is fairly under-documented. Therefore, this study was aimed to detect the presence of three common avian viruses that infect psittacine birds, i.e. beak and feather disease virus (BFDV), avian polyomavirus and avian papillomavirus.
Methodology and results:Faecal samples from twelve asymptomatic captive psittacine birds of different species were collected from an undisclosed animal garden in Serdang, Selangor, Malaysia. Briefly, the sample was homogenised and resuspended with SM buffer with the ratio 1:1 (weight of sample/g: volume of SM buffer/mL) before centrifugation at 1,000 × g for 20 min. The supernatant was collected and filtered before subjected to genomic DNA extraction using a commercialised kit. Polymerase chain reaction (PCR) technique was used to screen the V1, VP1 and L1 genes of beak and feather disease virus (BFDV), avian polyomavirus and avian papillomavirus, respectively. Findings revealed that the samples were negative for BFDV and avian polyomavirus. However, positive results of 1.5 kbp PCR amplicon were detected for avian papillomavirus in four out of the 12 samples (33.33%), which was from the white-crested cockatoo, African grey parrot, yellow-collared macaw and Senegal parrot. Sequence analysis of the L1 gene from the Senegal parrot Poicephalus senegalus revealed 93% identity to a reference Psittacus erithacus timneh avian papillomavirus.
Conclusion, significance and impact of study:This study added to the limited prevalence data of three important avian viruses which infect captive psittacines in Seri Kembangan, Selangor, Malaysia. Avian papillomavirus, but not BFDV and avian polyomavirus, was detected in the collected captive psittacine birds. Therefore, a routine screening can be performed to monitor the health status of birds despite their asymptomatic manifestation, in order to prevent possible virus transmission.
Full text:20.2021my0036.pdf