Adherence and internalisation of Lactococcus lactis M4 towards human colorectal cancer cell line, Caco-2
- Author:
Hanis Faudzi
1
;
Suet Lin Chia
2
,
3
;
Raha Abdul Rahim
1
;
Sarah Othman
3
,
4
Author Information
- Publication Type:Other Types
- Keywords: Lactococcus lactis; Adherence; Internalisation; Caco-2
- MeSH: Lactococcus lactis; Colorectal Neoplasms; Caco-2 Cells
- From:Malaysian Journal of Microbiology 2021;17(3):321-325
- CountryMalaysia
- Language:English
-
Abstract:
Aims:Lactococcus lactis is a non-colonizing, generally-regarded as safe (GRAS) lactic acid bacteria that has been
frequently studied as a potential vector for bactofection. To mediate bactofection, a series of interaction between the
bacteria and the host cell needs to occur. This study aims to investigate the in vitro bacterial-cell interaction between a
locally-isolated L. lactis M4 strain with human colorectal cancer line, Caco-2.
Methodology and results:Bacterial interaction was evaluated via adherence and internalisation assays. A 250:1 ratio of bacteria to cancer cell was selected as the optimum multiplicity of infection for all assays. After 2 h, L. lactis M4 was able to adhere to and internalise into Caco-2 cells at comparable rates to commercial strains L. lactis NZ9000 and MG1363.
Conclusion, significance and impact of study:Findings from this study showed that this strain has similar interaction properties with the commercial strains and would make a promising candidate for future bactofection studies and development of bacteria-mediated DNA vaccination against various diseases. - Full text:20.2021my0033.pdf
