Isolation and characterization of Lactobacillus brevis C23 with ability to secrete antimicrobial substance for the inhibition of a foodborne pathogen Listeria monocytogenes ATCC 7644

Dharni Kuhan Sreedharan; Sahar Abbasiliasi; Paramasivam Murugan; Zhang Jin Ng; Arbakariya Bin Ariff; Joo Shun Tan

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Isolation and characterization of Lactobacillus brevis C23 with ability to secrete antimicrobial substance for the inhibition of a foodborne pathogen Listeria monocytogenes ATCC 7644

Author: Dharni Kuhan Sreedharan ¹ ; Sahar Abbasiliasi ² ; Paramasivam Murugan ¹ ; Zhang Jin Ng ¹ ; Arbakariya Bin Ariff ^{3
,

4} ; Joo Shun Tan ⁵
Author Information

1. School of Industrial Technology, Universiti Sains Malaysia, 11800 Gelugor, Pulau Pinang, Malaysia.
2. Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
3. Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia UPM, Serdang 43400, Selangor, Malaysia &
4. Bioprocessing and Biomanufacturing Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia UPM, Serdang 43400, Selangor, Malaysia
5. School of Industrial Technology, Universiti Sains Malaysia, 11800 Gelugor, Pulau Pinang, Malaysia
Publication Type:Journal Article
Keywords: Lactic acid bacteria; Lactobacillus brevis; Foodborne pathogen; Listeria monocytogenes; Antimicrobial
MeSH: Lactobacillales; Listeria monocytogenes; Foodborne Diseases; Sprains and Strains
From:Malaysian Journal of Microbiology 2021;17(3):266-276
CountryMalaysia
Language:English
Abstract: Aims:This study aims to isolate lactic acid bacteria (LAB) from various food sources to obtain a potent strain against Listeria monocytogenes.
Methodology and results:A total of 68 LAB isolates were selected to evaluate their antimicrobial activity against L. monocytogenes, a foodborne pathogen and a causative agent of listeriosis. The selected isolate was identified and characterized. The isolate C23 from cabbage showed the highest antimicrobial activity against L. monocytogenes ATCC 7644 with inhibition ability of 73.94%. The isolate was closely related to Lactobacillus brevis by 16S rRNA sequencing and subsequently deposited in GenBank with an accession number of MN880215, named as L. brevis C23. The cell free supernatant (CFS) of L. brevis C23 had high tolerance in low pH and was able to withstand up to 60 °C. The proteinaceous nature of the antimicrobial agent was also confirmed through the enzymatic test. The CFS was stable on different detergents as well as bile salts. Under transmission electron microscopy (TEM), the inhibitory effect of CFS against L. monocytogenes was proven by causing cell lysis.
Conclusion, significance and impact of study:Bacteriocin-like inhibitory substances (BLIS) of L. brevis C23 showed very promising potential in food industrial application.
Full text:20.2021my0027.pdf