The use of phosphorus nanoparticles synthesized by rhizospheric fungus Aspergillius fumigatus as a nanofertilizer for flax plant
- Author:
Rabaa Yaseen
1
;
Basma Hamdy Amin
2
Author Information
- Publication Type:Journal Article
- Keywords: Nano-phosphorus; Biosynthesis; Cytotoxicity; Leaf ultrastructure
- MeSH: Nanoparticles--chemistry; Aspergillus fumigatus
- From:Malaysian Journal of Microbiology 2021;17(3):244-253
- CountryMalaysia
- Language:English
-
Abstract:
Aims:This study examined the mycosynthesis of phosphorus nanoparticles (PNPs) and its application as a fertilizer for flax plant.
Methodology and results:A total of thirty eight fungal isolates were isolated and screened for their abilities to synthesize PNPs. The fungal isolate was determined and identified as Aspergillus fumigatus (NCBI GenBank accession No. MN610566-MN610567). The biosynthesized nanoparticles were characterized by particle size analyzer, UV-visible spectrophotometer, transmission electron microscope (TEM), energy-dispersive X-ray spectroscopy (EDX) and fourier transform infrared spectroscopy (FT-IR). They were found to have an average diameter of 45.1 nm, regular round shape, EDX confirms the 54.63 atom % of phosphorous. The cytotoxicity of produced nanoparticles was performed to determine the safe dose that will be applied in agricultural experiment and was found to be 12.5 μg/mL. Pot experiment was performed to determine the fertilizing impact of mycosynthesized PNPs on flax plant and to equate their influence with granular single super phosphate. Results revealed that growth parameters, phosphorus content and microbial activities in the rhizosphere of flax plants were highly significantly (p ≤ 0.05) affected by foliar application of PNPs in presence of half dose of super phosphate. The TEM-micrographs of stained ultrastructural leaves showed that the PNPs treated leaves in the presence of half dose of super phosphate had normal cell structure similar to control, while the cell structure of leaves treated with PNPs but did not receive super phosphate were adversely affected.
Conclusion, significance and impact of study:This study clearly indicated that the application of low cost biosynthesised PNPs could save about 50% of recommended dose of phosphorus fertilizer. This study also demonstrates that it is not preferred to use PNPs as a fertilizer alone without adding super phosphate. Hence, this investigation suggests that further studies should be established to detect the safety of this nanofertilizers. - Full text:20.2021my0025.pdf
