Molecular epidemiology of piliated pneumococcal isolates at a major tertiary hospital in the Klang Valley, Malaysia

Nurul Dzaraly; Mohd Desa; Abdulrahman Muthanna; Nurul Baharin; Nurshahira Sulaiman; Nurul Rahman; Siti Masri; Niazlin Taib; Semiat Yusuff; Nurul Rahman; Navindra Palanisamy; Zarizal Suhaili; Nor Rahman; Tuan Soh; Fatimah Abdullah8

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Molecular epidemiology of piliated pneumococcal isolates at a major tertiary hospital in the Klang Valley, Malaysia

Author: Nurul DZARALY ¹ ; Mohd DESA ¹ ; AbdulRahman MUTHANNA ¹ ; Nurul BAHARIN ¹ ; Nurshahira SULAIMAN ¹ ; Nurul RAHMAN ¹ ; Siti MASRI ² ; Niazlin TAIB ² ; Semiat YUSUFF ² ; Nurul RAHMAN ³ ; Navindra PALANISAMY ⁴ ; Zarizal SUHAILI ⁵ ; Nor RAHMAN ⁶ ; Tuan SOH ⁷ ; Fatimah ABDULLAH8 ⁸
Author Information

1. Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Upm Serdang
2. Department of Medical Microbiology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Upm Serdang
3. School of Biology, Faculty of Applied Sciences, Universiti Teknologi Mara Negeri Sembilan, Kuala Pilah Branch
4. Department of Medical Microbiology and Parasitology, Faculty of Medicine, Universiti Teknologi Mara, Jalan Hospital
5. School of Animal Science, Aquatic Science and Environment, Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin
6. Faculty of Medicine, Universiti Sultan Zainal Abidin
7. Department of Pathology, Sungai Buloh Hospital, Jalan Hospital
8. Department of Pathology, Sultanah Nur Zahirah Hospital
Publication Type:Journal Article
From: Asian Pacific Journal of Tropical Medicine 2023;16(2):80-88
CountryChina
Language:Chinese
Abstract: Objective: To characterise a collection of pili-carrying and none pili-carrying pneumococcal isolates of clinical origin for serotypes, antibiotic resistance and genotype. Methods: In total, 42 clinical isolates were collected between October 2017 and December 2019. Those isolates were analysed for antimicrobial susceptibility, serotype distribution, detection of pneumococcal virulence and pilus genes. Multilocus sequence typing was performed only for piliated isolates, followed by phylogenetic analysis. Results: The common isolation sites among the pneumococcal isolates were tracheal aspirate (28.6%), blood (26.2%), and sputum (23.8%). Fifty percent isolates were resistant to erythromycin, tetracycline (50.0%) and trimethoprim-sulfamethoxazole (43.0%). The most frequent were serotypes 19F (28.6%), 6A/B (23.8%) and 19A (14.3%). Piliated isolates were detected in a small proportion (33.3%); 64.3% were multidrug-resistant. ST320 was the prevalent sequence type among the piliated isolates and genetically related to the Pneumococcal Molecular Epidemiology Network clones Taiwan 19F -14 (CC271). In the phylogenetic analysis, some piliated isolates showed a close association having similar ST320, carrying serotype 19A and both pilus genes indicating their clonal spread. Conclusions: Pneumococcal lineages of piliated isolates have been globally disseminated and pili could have played a role in the spread of antibiotic resistant clones.