Effect of PPARγ agonist (rosiglitazone) on the secretion of Th2 cytokine in asthma mice
10.1016/j.apjtm.2016.10.006
- Author:
Ji-Hui YU
1
;
Li LONG
1
;
Zhi-Xiao LUO
1
;
Jie-Ru YOU
1
Author Information
1. Department of Physical Examination, The First Affiliated Hospital of Chongqing Medical University
- Publication Type:Journal Article
- Keywords:
Asthma;
PPARγ;
Th17;
Th2;
Treg
- From:
Asian Pacific Journal of Tropical Medicine
2017;10(1):64-68
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of PPARγ agonist (rosiglitazone) on the secretion of Th2 cytokines and the proportion of immune cell subsets in asthma mice. Methods Ovalbumin (OVA)-sensitized mice were used to build asthma models. Those mice were divided into the normal control group, model group and rosiglitazone group. Differences of the changes in lung histopathology of mice in the three groups were observed through hematoxylin and eosin (HE) strain, and the numbers of the total cells, eosinophils and neutrophils in BALF of mice in the three groups were compared. ELISA and real-time PCR were employed to detect the protein levels of interleukin (IL)-5, IL-13, IL-4 and IL-10 and mRNA level, respectively. Flow cytometry number was implied to analyze the proportion of immune cell subsets in peripheral blood of mice. Results Compared with the mice in the control group, and mice of the model group, the infiltration of inflammatory cells in BALF increased, bronchial smooth muscle became thickened, a large amount of collagen deposited, the secretion of Th2 cytokine increased significantly, the ratio of regulatory T cells (Treg) decreased, the ratio of T17 cells rose distinctly; while in mice of the rosiglitazone group, the changes of their lung histopathology were improved obviously, the number of infiltration of inflammatory cells declined, the thickened smooth muscle relieved, the deposition of collagen decreased, the secretion of Th2 cytokine was inhibited, the ratio of Treg went up, and the increased of the ratio of T17 cells was inhibited but still not return to normal level. Conclusions Rosiglitazone can regulate the proportion of Treg and Th17 cells and inhibit the secretion of Th2 cytokines, which inhibit the airway inflammatory response for asthma mice effectively.
