Pyrrolidine dithiocarbamate alleviates the anti-tuberculosis drug-induced liver injury through JAK2/STAT3 signaling pathway: An experimental study
10.1016/j.apjtm.2017.05.010
- Author:
Hong ZHANG
1
;
Yang LIU
2
;
Li-Kun WANG
3
;
Na WEI
4
Author Information
1. Internal Medicine Department No. 2, Linyi Chest Hospital in Shandong Province
2. Child Healthcare Department, Linyi Chest Hospital in Shandong Province
3. Infectious Disease Department No. 6 Ward, Linyi People's Hospital in Shandong Province
4. Laboratory Department, Linyi Chest Hospital in Shandong Province
- Publication Type:Journal Article
- Keywords:
Anti-tuberculosis drug;
Drug-induced liver injury;
JAK2;
Pyrrolidine dithiocarbamate;
STAT3
- From:
Asian Pacific Journal of Tropical Medicine
2017;10(5):520-523
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of pyrrolidine dithiocarbamate (PDTC) on the anti-tuberculosis drug-induced liver injury and the molecular mechanism. Methods Clean male SD rats were selected as experimental animals and randomly divided into normal group, model group, PDTC group and AG490 group. Animal model of anti-tuberculosis drug-induced liver injury was established by intragastric administration isoniazid + rifampicin. PDTC group received intraperitoneal injection of PDTC, and AG490 group received intraperitoneal injection of AG490. Twenty-eight days after intervention, the rats were executed, and the liver injury indexes, inflammation indexes and oxidative stress indexes in serum as well as JAK2/STAT3 expression, liver injury indexes, inflammation indexes and oxidative stress indexes in liver tissue were determined. Results p-JAK2, p-STAT3, TNF-α, IL-1β, IL-6, ROS, 8-OHdG and MDA expression in liver tissue as well as TBIL, ALT, AST, γ-GT, TNF-α, IL-1β, IL-6, 8-OHdG and MDA levels in serum of model group were significantly higher than those of normal group while p-JAK2, p-STAT3, TNF-α, IL-1β, IL-6, ROS, 8-OHdG and MDA expression in liver tissue as well as TBIL, ALT, AST, γ-GT, TNF-α, IL-1β, IL-6, 8-OHdG and MDA levels in serum of PDTC group and AG490 group were significantly lower than those of model group. Conclusions PDTC can inhibit the inflammation and oxidative stress mediated by JAK2/STAT3 signaling pathway to alleviate the anti-tuberculosis drug-induced liver injury.
