Preoperative application of combination of portal venous injection of donor spleen cells and intraperitoneal injection of rapamycin prolongs the survival of cardiac allografts in mice
10.1016/j.apjtm.2017.05.004
- Author:
Wen-lin GONG
1
;
Gang DU
1
;
Su-fang ZHOU
1
;
Nuo YANG
1
;
Yong-xiang ZHAO
1
;
Chuang SHA
2
;
Zhong-gui SHAN
3
;
Zhong-quan QI
3
;
Nuo YANG
4
;
Yong-xiang ZHAO
4
Author Information
1. National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University
2. Department of Cardiac Surgery, Zhongshan Hospital, Xiamen University
3. Organ Transplantation Institute, Xiamen University
4. Department of Cardio-Thoracic Surgery, The First Affiliated Hospital of Guang Xi Medical University
- Publication Type:Journal Article
- Keywords:
Heart transplantation tolerance;
Portal venous injection of donor spleen cells;
Rapamycin
- From:
Asian Pacific Journal of Tropical Medicine
2017;10(5):454-460
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of preoperative portal venous injection of donor spleen cells (PVIDSC) and intraperitoneal injection of rapamycin in the acute rejection of cardiac allograft in mice and the underlying mechanisms. Methods Homogenous female B6 mice and BALB/c mice were used as recipients and donors of heart transplantation. These mice were randomly divided into different groups and received PVIDSC alone, rapamycin alone, or PVIDSC and rapamycin combined therapy. In addition, the underlying mechanism was studied by measuring a number of cytokines. Results Preoperative combination of PVIDSC and intraperitoneal injection of rapamycin significantly prolonged the survival of heterotopic cardiac allograft in mice, but had no effects on the survival time of cardiac allografts in mice pre-sensitized by skin grafting. Preoperative combination of PVIDSC and intraperitoneal injection of rapamycin increased the expression of IL-10 and Foxp3 and reduced the expression of INF-. Short-term preoperative administration of rapamycin promotes the expression of CD4
