First report on molecular characterization of Leishmania species from cutaneous leishmaniasis patients in southern Khyber Pakhtunkhwa province of Pakistan
10.1016/j.apjtm.2017.07.015
- Author:
Mubbashir HUSSAIN
1
;
Bahar Ullah KHATTAK
1
;
Taj Ali KHAN
1
;
Niaz MUHAMMAD
1
;
Muhammad ANEES
1
;
Hazir RAHMAN
1
;
Muhammad QASIM
1
;
Humaira MAZHAR
1
;
Shahzad MUNIR
2
;
Sultan AYAZ
3
;
Muhammad Ameen JAMAL
4
;
Irfan AHMED
5
;
Kashif RAHIM
6
;
Noha WATANAY
7
;
Mohamed KASBARI
8
Author Information
1. Vector Borne Diseases Laboratory, Department of Microbiology, Kohat University of Science and Technology
2. Department of Plant Pathology, Faculty of Plant Protection, Yunnan Agricultural University
3. College of Animal Husbandry and Veterinary Sciences, Abdul Wali Khan University Mardan
4. College of Animal Science and Technology, Yunnan Agricultural University
5. Yunnan Provincial Key Laboratory of Animal Nutrition and Feed, Yunnan Agricultural University
6. Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University
7. US Naval Medical Research Unit-3, (NAMRU 3)
8. French Agency for Health and Safety Animal Health Laboratory
- Publication Type:Journal Article
- Keywords:
Cutaneous leishmaniasis;
ITS-PCR;
Khyber Pakhtunkhwa;
Leishmania tropica;
RFLP
- From:
Asian Pacific Journal of Tropical Medicine
2017;10(7):718-721
- CountryChina
- Language:Chinese
-
Abstract:
Objective To report presence of Leishmania major in Khyber Pakhtunkhwa of Pakistan, where cutaneous leishmaniasis (CL) is endemic and was thought to be caused by Leishmania tropica only. Methods Biopsy samples from 432 CL suspected patients were collected from 3 southern districts of Khyber Pakhtunkhwa during years 2011–2016. Microscopy on Giemsa stained slides were done followed by amplification of the ribosomal internal transcribed spacer 1 gene. Results Leishmania amastigotes were detected by microscopy in 308 of 432 samples (71.3%) while 374 out of 432 samples (86.6%) were positive by ribosomal internal transcribed spacer 1 PCR. Subsequent restriction fragment length polymorphism confirmed L. tropica in 351 and L. major in 6 biopsy samples. Conclusions This study is the first molecular characterization of Leishmania species in southern Khyber Pakhtunkhwa. It confirmed the previous assumptions that anthroponotic CL is the major CL form present in Khyber Pakhtunkhwa province. Furthermore, this is the first report of L. major from a classical anthroponotic CL endemic focus identified in rural areas of Kohat district in southern Khyber Pakhtunkhwa.
