Measurement of total phenolic content and antioxidant activity of aerial parts of medicinal plant Coronopus didymus
10.1016/j.apjtm.2017.07.024
- Author:
Hafiza NOREEN
1
;
Muhammad FARMAN
1
;
Nabil SEMMAR
2
;
James S.O. MCCULLAGH
3
Author Information
1. Department of Chemistry, Quaid-i-Azam University
2. Institut Supérieur des Sciences Biologiques Appliquées de Tunis
3. Chemistry Research Laboratory, Department of Chemistry, University of Oxford
- Publication Type:Journal Article
- Keywords:
Antioxidant activity;
Coronopus didymus;
Size exclusion chromatography;
Total phenolic content
- From:
Asian Pacific Journal of Tropical Medicine
2017;10(8):792-801
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the total phenolic content and compare the antioxidant activity of various solvent extracts and fractions from the aerial parts of Coronopus didymus through various assays. Methods Total phenolic content was determined using the Folin-Ciocalteu assay and the in vitro antioxidant activity of a number of different extracts was investigated in a dose-dependent manner with three different methods: the 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and ferric reducing antioxidant power (FRAP) assays. A flavone was isolated from the most active ethanolic extract with high antioxidant activity using size exclusion chromatography. IC
