Biomass accumulation of Panax vietnamensis in cell suspension cultures varies with addition of plant growth regulators and organic additives
10.1016/j.apjtm.2017.08.012
- Author:
Tuan Tran TRONG
1
;
Dieu-Thai TRAN
1
;
Huyen-Trang NGUYEN THI
1
;
Dieu-Hien TRUONG
2
;
Hoang Chinh NGUYEN
2
;
Giap Do DANG
2
;
Ho Nguyen HUU
3
Author Information
1. Plant Cell Technology Department, Institute of Tropical Biology, Vietnam Academy of Science and Technology
2. Faculty of Applied Sciences, Ton Duc Thang University
3. Genetic Engineering Department, Institute of Tropical Biology, Vietnam Academy of Science and Technology
- Publication Type:Journal Article
- Keywords:
Biomass;
Casein hydrolyzate;
Panax vietnamensis;
Plant cell suspension culture;
Plant regulators;
Yeast extract
- From:
Asian Pacific Journal of Tropical Medicine
2017;10(9):907-915
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the impact of plant growth regulators including kinetin (KN), benzyl adenine and naphthalene acetic acid, yeast extract and casein hydrolyzate on biomass accumulation of Vietnamese ginseng Panax vietnamensis (P. vietnamensis) in cell suspension culture. Methods Cell suspension cultures were established from friable calluses derived from leaves and petioles of 3-year-old in-vitro P. vietnamensis plants. The cell suspension cultures were grown in Murashige and Skoog basal media supplemented with various concentrations of KN, benzyl adenine, naphthalene acetic acid, and yeast extract and casein hydrolyzate. Results All tested factors generated an increase in the cell biomass of P. vietnamensis in suspension culture, but the impact of each varies depended on the factor type, concentration, and incubation period. Addition of 2.0 mg/L KN resulted in the largest biomass increase after 24 d, (57.0 ± 0.9) and (3.1 ± 0.1) mg/mL fresh and dry weight, respectively, whereas addition of benzyl adenine or naphthalene acetic acid produced optimum levels of Panax cell biomass at 1.0 and 1.5 mg/L, respectively. Addition of the elicitor yeast extract led to a 1.4–2.4 fold increase in biomass of P. vietnamensis, while addition of casein hydrolyzate enhanced biomass accumulation 1.8–2.6 fold. Conclusions The addition of each factor causes significant changes in biomass accumulation of P. vietnamensis. The largest biomass accumulation is from cultures grown in MS media containing 2.0 mg/L KN for 24 d. The outcome of the present study provides new insights into the optimal suspension culture conditions for studies on the in vitro cell biomass production of P. vietnamensis.
