Clinical and environmental isolates of Burkholderia pseudomallei from Brazil: Genotyping and detection of virulence gene
10.1016/j.apjtm.2017.09.004
- Author:
Tereza de Jesus Pinheiro Gomes BANDEIRA
1
;
Débora de Souza Collares Maia CASTELO-BRANCO
1
;
Marcos Fábio Gadelha ROCHA
1
;
Rossana de Aguiar CORDEIRO
1
;
Crister José OCADAQUE
1
;
Manoel de Araújo Neto PAIVA
1
;
Raimunda Sâmia Nogueira BRILHANTE
1
;
José Júlio Costa SIDRIM
1
;
Tereza de Jesus Pinheiro Gomes BANDEIRA
2
;
Marcos Fábio Gadelha ROCHA
3
;
Manoel de Araújo Neto PAIVA
4
Author Information
1. Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Federal University of Ceará
2. Christus School of Medicine
3. Postgraduate Program in Veterinary Sciences, State University of Ceará
4. Federal Institute of Education, Science and Technology of Ceará
- Publication Type:Journal Article
- Keywords:
B. pseudomallei;
Melioidosis;
RAPD;
TTSS gene
- From:
Asian Pacific Journal of Tropical Medicine
2017;10(10):945-951
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the genetic diversity of clinical and environmental isolates of Burkholderia pseudomallei (B. pseudomallei) recovered in Ceará Brazil, and screen these isolates for the presence of type three secretion system virulence gene. Methods Nineteen B. pseudomallei isolates (9 from clinical cases and 10 from soils) were analyzed. Random amplified polymorphic DNA was performed with primers OPQ-2, OPQ-4 and OPQ-16 to evaluate the genetic diversity, and type three secretion system gene was detected through polymerase chain reaction. Results Random amplified polymorphic DNA showed a genetic relatedness of approximately 50% among the tested B. pseudomallei isolates, which were grouped into two clades, of which the biggest ones comprised 18/19 isolates for primer OPQ-2, and 17/19 isolates for primer OPQ-16. Primer OPQ-4 grouped the isolates into three clades comprising 1/19, 3/19 and 15/19 isolates. Additionally, type three secretion system gene was detected in all tested isolates. Conclusions This was an effort to type B. pseudomallei strains from Ceará which is important for better understanding this pathogen, contributing for the epidemiological surveillance of melioidosis in this endemic region.
