Glypican-3-specific cytotoxic T lymphocytes induced by human leucocyte antigen-A*0201-restricted peptide effectively kill hepatocellular carcinoma cells in vitro

Author: Jiang-Zheng ZENG ¹ ; Rong-Cheng LUO ¹ ; Jiang-Zheng ZENG ² ; Fen HUANG ² ; Zhi-Hui HE ² ; Huamao SUN ² ; Yan-Da LU ² ; Jun-Hua LEI ² ; Yu LIU ³
Author Information

1. Cancer Center, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University
2. Department of Medical Oncology, The First Affiliated Hospital of Hainan Medical College, Hainan Medical College Cancer Institute
3. Department of Breast and Thoracic Tumor Surgery, The First Affiliated Hospital of Hainan Medical College
Publication Type:Journal Article
Keywords: Cytotoxic T lymphocyte; Glypican-3; Hepatocellular carcinoma; Peptide
From: Asian Pacific Journal of Tropical Medicine 2017;10(11):1084-1089
CountryChina
Language:Chinese
Abstract: Objective To investigate potential human leucocyte antigen (HLA)-A2-restricted epitope peptides of glypican-3 (GPC3) and determine the cytotoxicity of peptide-specific cytotoxic T lymphocytes (CTLs) against hepatocellular carcinoma (HCC) cells. Methods The potential HLA-A*0201-restricted GPC3 peptides were screened using computer algorithms, T2 cell-binding affinity and stability of peptide/HLA-A*0201 complex assay. The peptide-specific CTLs were generated and their cytotoxicity against GPC3