Reverse effect of curcumin on CDDP-induced drug-resistance via Keap1/p62-Nrf2 signaling in A549/CDDP cell
- Author:
Jie SHEN
1
;
Yuan-Wei JIA
1
;
Jie SHEN
2
;
Ya-Juan CHEN
2
;
Yuan-Wei JIA
2
;
Guang-Hai CHEN
2
;
Ding-Feng LIU
2
;
Yun-Yu CHEN
2
;
Chao ZHANG
2
;
Xiao-Ping LIU
2
;
Jie SHEN
3
;
Yuan-Wei JIA
3
;
Jie SHEN
4
;
Yun-Yu CHEN
4
;
Chao ZHANG
4
;
Xiao-Ping LIU
4
;
Wen-Ying ZHAO
5
Author Information
- Publication Type:Journal Article
- Keywords: Autophagy; Curcumin; Drug-resistance; Keap1; Nrf2
- From: Asian Pacific Journal of Tropical Medicine 2017;10(12):1190-1196
- CountryChina
- Language:Chinese
- Abstract: Objective To assess the effect of curcumin on CDDP-induced drug resistance and explore the underlying molecular mechanism through Nrf2 system and autophagy pathway. Methods A drug-resistant cell model was established by exposing A549/CDDP cell to 2 μg/mL CDDP. A549/CDDP cell was treated with 20 μg/mL CDDP and 10 μM curcumin. The cell viability and apoptosis level, the signals of Keap1/P62-Nrf2 and autophagy pathway were analyzed. Results CDDP induction promoted drug-resistant phenotype in A549/CDDP cell and activated autophagy as well as Nrf2 signals in A549/CDDP cell. Meanwhile, curcumin combination attenuated autophagy and Nrf2 activation induced by CDDP, and reversed the drug-resistant phenotype. Notably, curcumin combination augmented Keap1 transcription. Furthermore, Keap1 ablation with short hairpin RNAs hampered the efficacy of curcumin, suggesting Keap1 played a crucial role on reversal effect of curcumin. Conclusions The present findings demonstrate that CDDP promotes abnormal activation of Nrf2 pathway and autophagy, leading to drug resistance of A549/CDDP cell. Curcumin attenuates this process and combat drug-resistance through its potent activation on Keap1 transcription, which is essential for interplay between oxidative stress induced Nrf2 activation and autophagy/apoptosis switch.
