Development of a mouse model of arecoline-induced oral mucosal fibrosis
10.1016/j.apjtm.2017.10.026
- Author:
Qi-Tao WEN
1
;
Tao WANG
1
;
Da-Hai YU
2
;
Zheng-Rui WANG
3
;
Ying SUN
3
;
Cui-Wei LIANG
4
Author Information
1. Dental Medical Center, People's Hospital of Hainan Province
2. Department of Stomatology, First Affiliated Hospital of Guangxi Medical University
3. Department of Oral and Maxillofacial Surgery, Stomatology Hospital, Guangxi Medical University
4. Department of Oral Medicine, Haikou City People's Hospital
- Publication Type:Journal Article
- Keywords:
Angiogenesis;
Arecoline;
Collagen;
Mouse model;
Oral submucous fibrosis
- From:
Asian Pacific Journal of Tropical Medicine
2017;10(12):1177-1184
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a BALB/c mouse model of oral submucous fibrosis (OSF) induced by arecoline and to exhibit an accumulation of collagen and angiogenesis changes. Methods BALB/c mice were randomly assigned to either the control (distilled water) or experimental group (arecoline) (n = 40). Eight mice from each group were sacrificed every 4 weeks since 8 weeks post treatment. Changes in histopathologic features, levels of collagen type I and collagen type III, and angiogenesis were measured. Results In the 8th week, epithelium atrophy, collagen cumulation and micrangium pathologic changes in the lamina propria were observed in the oral mucosa. In the 20th week, hyaline degeneration of the connective tissues was observed on the tongue and palate mucosa. The angiogenesis and collagen type I changed significantly as the diseases advanced (P < 0.05); however, collagen type III was not statistically different. Conclusions An OSF model involving mice can be rapidly induced by drinking a high-dose of arecoline. OSF angiogenic changes in mice primarily decrease and collagen accumulation is mainly collagen type I.
