Chloride channel involved in the regulation of curcumin-induced apoptosis of human breast cancer cells
- Author:
You-Wei HUANG
1
;
Jia-Hong CHEN
1
;
Zi-Xi QIN
1
;
Jie-Kun CHEN
1
;
Ren-Dong HU
1
;
Xi LIN
1
;
Zheng WU
2
;
Zheng WU
3
Author Information
- Publication Type:Journal Article
- Keywords: Apoptosis; Chloride channel 3; Combination; Curcumin; DIDS; Mcf-7
- From: Asian Pacific Journal of Tropical Medicine 2018;11(3):240-244
- CountryChina
- Language:Chinese
- Abstract: Objective: To investigate the role of ClC-3 chloride channel in the proliferation of breast cancer cell line Mcf-7 treated with curcumin and its specific mechanism. Methods: MTT assay was used to detect the effect of chloride channel blocker (DIDS) and curcumin on Mcf-7 and human normal cell viability. Patch-clamp technique was used to determine the current density before and after drug treatment. Apoptosis assay by flow cytometry was performed for further examination of cell apoptosis. Results: Curcumin had toxicity on Mcf-7 and HUVEC cells and DIDS reduced the survival rate of Mcf-7 cells by inhibiting proliferation. Curcumin could activate the chloride ion current on MCF-7 cell membrane, which would be inhibited by DIDS. Finally, curcumin in low concentration combined with DIDS could significantly promote the MCF-7 cells apoptosis. Conclusions: Our results suggest that ClC-3 protein is involved in the regulation of curcumin induced proliferation inhibiting in breast cancer cells through inducing cell apoptosis. ClC-3 may be a potential target of tumor therapy.
