Inhibitory activities of extracts of Rumex dentatus, Commelina benghalensis, Ajuga bracteosa, Ziziphus mauritiana as well as their compounds of gallic acid and emodin against dengue virus
- Author:
Riffat BATOOL
1
;
Ejaz AZIZ
1
;
Tariq MAHMOOD
1
;
Benny TAN
2
;
Vincent CHOW
3
Author Information
- Publication Type:Journal Article
- Keywords: Ajuga bracteosa; Commelina benghalensis; Dengue virus; Gallic acid; Rumex dentatus; Ziziphus mauritiana
- From: Asian Pacific Journal of Tropical Medicine 2018;11(4):265-271
- CountryChina
- Language:Chinese
- Abstract: Objective: To investigate the inhibitory effects against dengue virus serotype 2 (DENV-2) by five different fractions (extracted by methanol, ethanol, benzene, chloroform and n-hexane) of Rumex dentatus, Commelina benghalensis, Ajuga bracteosa and Ziziphus mauritiana, as well as their constituents (gallic acid, emodin, and isovanillic acid). Methods: All the samples were tested for cytotoxicity on baby hamster kidney cells by MTT assay and for anti-DENV-2 activity by plaque reduction neutralization assay using two DENV-2 doses (45 and 90 plaque- forming units or PFU). Results: All the samples except isovanillic acid exhibited significant prophylactic effects against DENV-2 infectivity (without cytotoxicity) when administered to cells before infection, but were not effective when given 6 h post-infection. The methanol extract of Rumex dentatus demonstrated the highest antiviral efficacy by inhibiting DENV-2 replication, with IC
