Inhibitory activities of plumbagin on cell migration and invasion and inducing activity on cholangiocarcinoma cell apoptosis

Luxsana Panrit; Luxsana Panrit; Tullayakorn Plengsuriyakarn; Pongsakorn Martviset; Kesara Na-bangchang; Tullayakorn Plengsuriyakarn; Kesara Na-bangchang; Pongsakorn Martviset

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Inhibitory activities of plumbagin on cell migration and invasion and inducing activity on cholangiocarcinoma cell apoptosis

Author: Luxsana PANRIT ¹ ; Luxsana PANRIT ² ; Tullayakorn PLENGSURIYAKARN ² ; Pongsakorn MARTVISET ² ; Kesara NA-BANGCHANG ² ; Tullayakorn PLENGSURIYAKARN ³ ; Kesara NA-BANGCHANG ³ ; Pongsakorn MARTVISET ⁴
Author Information

1. Drug Discovery and Development Center, Thammasat University
2. Center of Excellence in Molecular Biology and Pharmacology of Malaria and Cholangiocarcinoma, Chulabhorn International College of Medicine, Thammasat University
3. Graduate Program in Bioclinical Sciences, Chulabhorn International College of Medicine, Thammasat University
4. Faculty of Medical Technology, Rangsit University
Publication Type:Journal Article
Keywords: Apoptosis; Cancer invasion; Cancer migration; Cholangiocarcinoma; Plumbagin
From: Asian Pacific Journal of Tropical Medicine 2018;11(7):430-435
CountryChina
Language:Chinese
Abstract: Objective: To investigate the cytotoxic, apoptotic and inhibitory activities on cell migration and invasion of plumbagin in the human cholangiocarcinoma (CCA) cell line (CL-6) in comparison with human embryonic fibroblast cell line (OUMS). Methods: Cytotoxicity activity was evaluated using MTT assay. Inhibitory effect on cell migration and invasion were investigated using label-free real-time cell analysis and QCM ECMatrix cell invasion chamber, respectively. Apoptotic activity was evaluated using flow cytometry and CellEvent™ Caspase 3/7 assay. Results: Based on results of the cytotoxicity test in CL-6 cells, 50% inhibitory concentration (IC