Optimization of extraction process of prescription medicinal materials of compound Yangshe granules
10.12206/j.issn.2097-2024.202207103
- VernacularTitle:复方羊蛇颗粒处方药材提取工艺的优化研究
- Author:
Jianchao YU
1
,
2
;
Chao MA
3
;
Mengmeng WANG
3
;
Dan LI
3
;
Rongmei LIU
2
;
Jiyong LIU
1
,
2
Author Information
1. Department of Pharmacy, Shanghai Cancer Center of Fudan University, Shanghai 200032, China
2. College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.
3. Department of Pharmacy, Shanghai Cancer Center of Fudan University, Shanghai 200032, China.
- Keywords:
compound Yangshe granule;
quantitative analysis;
extraction process;
central composite design-response surface methodology
- From:
Journal of Pharmaceutical Practice
2023;41(4):240-244
- CountryChina
- Language:Chinese
-
Abstract:
Objective Optimizing the extraction process of prescription medicinal materials of hospital preparation of compound Yangshe granules. Methods A high performance liquid chromatograph (HPLC) quantitative method was established for deacetyl asperulosidicacid methyl ester (DME) and ferulic acid (FC) of the active ingredient. Based on the content of DME, FC and the yield of extract, the extraction process of compound Yangshe granule extract was optimized using central composite design-response surface methodology. Results The established HPLC method of quantification of active components in compound Yangshe granules met the requirements of method validation. The optimal extraction process optimized by central composite design-response surface methodology were as follows: the weight of extraction solvent was 12 times of the medicinal slices, the alcohol concentration was 73% and the extraction time was 60 min. Conclusion In this study, the quantitative method of active components in compound Yangshe granule by HPLC has been successfully established, and the optimized extraction process is simple and easy to operate with good repeatability.
