Determination and effect of five alkaloids from extracts of Piper longum on rats with experimental myocardial ischemia induced by injection of pituitrin
10.12206/j.issn.2097-2024.202112011
- VernacularTitle:荜茇提取物中5个生物碱的含量测定与对垂体后叶素所致大鼠实验性心肌缺血的影响
- Author:
Bowen YI
1
;
Huining LIU
2
;
Rui ZHENG
1
;
Jiawei REN
3
;
Yang LIU
2
Author Information
1. Department of Pharmacy, Xiyuan Hospital, Chinese Academy of Traditional Chinese Medicine, Beijing 100091, China.
2. Beijing University of Chinese Medicine, Beijing 102488, China.
3. North China Electric Power University, Beijing 102206, China.
- Keywords:
extracts of Piper longum;
alkaloid;
content determination;
anti-myocardial ischemia
- From:
Journal of Pharmaceutical Practice
2023;41(4):218-221
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the content of five alkaloids from extracts of Piper longum and test the pharmacodynamic effect of them on rats with experimental myocardial ischemia induced by injection of pituitrin. Methods The content of five alkaloids was determined simultaneously by HPLC. The experimental myocardial ischemia in rats was induced by injection of pituitrin, and the absolute value of T wave change and change of heart rate before and after model establishment were chosen to be the observation index. The effects of large, medium and small dose groups were evaluated. Results Three batches of samples were analyzed, with the contents of piperine for 56.1%, 49.7%, 51.6%; N-isobutyl-(2E,4E)-octadecatrienamide for 4.5%, 4.2%, 4.3%; guineensine for 0.46%, 0.38%, 0.40%; piplartine for 1.73%, 1.67%, 1.70% and piperamide for 0.55%, 0.46%, 0.49%, respectively. All dose groups from extracts of piper longum had significantly reduced the absolute value of T wave and almost have no effect on the change of heart rate, except the high dose group showed the effect of reducing heart rate at some time . Conclusion The HPLC method was suitable for the simultaneous determination of five alkaloids from extracts of Piper longum. It was shown that extracts of Piper longum had good bioactivity in anti-myocardial ischemia.
