The Influences of Vascular Endothlelial Growth Factor and Endothelin-1 on Speramtogenesis in Testis.
- Author:
Sung Woo PARK
;
Hyun Jun PARK
;
Nam Cheol PARK
- Publication Type:Original Article
- Keywords:
Vascular endothelial growth factor;
Endothelin-1;
Spermatogenesis;
Testis
- MeSH:
Endothelin-1*;
Estradiol;
Germ Cells;
Humans;
Male;
Oligospermia;
Prolactin;
Seminiferous Tubules;
Sertoli Cell-Only Syndrome;
Spermatogenesis;
Testis*;
Vascular Endothelial Growth Factor A
- From:Korean Journal of Fertility and Sterility
2004;31(4):235-244
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: The effects on spermatogenesis by expression of vascular endothelial growth factor (VEGF) and endothelin-1 (ET-1) were investigated. MATERIALS AND METHODS: Testicular specimens were obtained from 40 infertile males due to primary testicular failure and from 10 fertile males with other urologic problems. The specimens of infertile males were devided into 4 groups according to histologic findings; Sertoli cell only syndrome (A), maturation arrest (B), hypospermatogenesis (C) and sloughing and disorganization (D). VEGF and ET-1 expression were detected with immunohistochemical stain. RESULTS: VEGF expression on Leydig cell was detected in all cases. But, VEGF expression rates on germ cell were significantly higher in infertile group B, C, D compared to that of the control group (p<0.05). ET-1 expression rates on Leydig cell was significantly lower in all infertile group compared to that of the control group (p<0.05). But, ET-1 expression rates on Sertoli cell was significantly higher in all infertile group compared to that of the control group (p>0.05). In germ cell of infertile group, LH, FSH and prolactin were significantly decreased, and estradiol is increased in positive stain group on ET-1 immunohistochemical stain (p<0.05). VEGF and ET-1 expression were not correlated mean seminiferous tubule diameter (p>0.05). CONCLUSIONS: Abnormal spermatogenesis would be reflected in VEGF expression in germ cell.
