Hypodermic of Glioma Cells into Nude Mice Inducing Glioma Model: Growth Characteristics Observation
- VernacularTitle:裸鼠皮下脑胶质瘤模型的复制与生长特性的观察
- Author:
Jingkun PAN
;
Hui ZHAO
;
Lei TIAN
;
Yun LUO
;
Yilong XUE
- Publication Type:Journal Article
- Keywords:
animal model, glioma, hypodermic inoculation, nude mouse
- From:
Chinese Journal of Rehabilitation Theory and Practice
2009;15(11):1019-1020
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the model of glioma by hypodermic of C6 cells line into the nude mice and observe the growth characteristics. Methods The C6 gliocyte suspension was injected into the left subaxillary hypoderma of nude mice. The diameter and the volume of the tumor were measured and calculated, and the volume-time growth curve was plotted. The cells of tumor were observed under the HE and glial fibrillary acidic protein (GFAP) immono-histochemistry staining. Results The tumor appeared in inoculation area 7 d after injection, and presented nearly exponential development after about 20 d. The survival time of the nude mice is 22～48 d. The tumors were with sharp border, affluent blood vessel, and a great number of GFAP-positive cells. Conclusion The model of glioma can be well induced by hypodermic of the cultured C6 gliocyte into nude mouse.
