Action Potential of Cardiomyocyte-like Cells Differentiated from Human Marrow Mesenchymal Stem Cells in Vitro
- VernacularTitle:人骨髓间充质干细胞体外定向诱导分化为心肌样细胞的动作电位观察
- Author:
Fangge DENG
;
Lianfeng GUO
;
Yingchao MA
;
Yulin LI
- Publication Type:Journal Article
- Keywords:
human mesenchymal stem cells (hMSCs), cardiomyocyte-like cells, patch clamp, action potential (AP), excitability
- From:
Chinese Journal of Rehabilitation Theory and Practice
2009;15(11):1014-1018
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect whether the cardiomyocyte-like cells differentiated from human marrow mesenchymal stem cells (hMSCs) could produce action potential (AP). Methods Isolated and cultured hMSCs were induced into cardiomyocyte-like cells with 5-Azacytine in vitro. They were measured for their AP by patch clamp technique, and compared with those of hMSCs of the same generation and beating cardiomyocytes (CMs) derived from 2 day-old SD rats. Results 6/30 cardiomyocyte-like cells produced AP. The CMs produced significant AP, hMSCs appeared no AP, and cardiomyocyte-like cells appeared weak AP. Conclusion The hMSCs manifested the potential to differentiate into CMs in the electrophysiology characteristics following 5-Azacytine induction.
