Phosphorylation and Protein Expression of p38 Mitogen Activated Protein Kinases in Cerebral Ischemic Preconditioning Rat
- VernacularTitle:脑缺血预适应大鼠脑内p38丝裂原激活蛋白激酶磷酸化水平和蛋白表达量的测定
- Author:
Lanfeng ZHAO
;
Shewei GUO
;
Yangyuan AN
;
Minggang LIU
- Publication Type:Journal Article
- Keywords:
cerebral ischemia preconditioning, p38 mitogen activated protein kinases(p38 MAPK), phosphorylation, protein expression, rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2008;14(9):801-803
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore initially the role of p38 mitogen activated protein kinases(p38 MAPK) in cerebral ischemic preconditioning.Methods Healthy adult SD rats were randomly divided into 5 groups: normal control group,sham-operated group,ischemia preconditioning or ischemia tolerance group,peripheral noxious control group,peripheral noxious tolerance group.SDS-PAGE,Western blot and Gel Doc imagine systems were applied to determine the p38 MAPK phosphorylation and protein expression in somatosensory cortex and hippocampus of rat.Results No significant changes of p38 MAPK in phosphorylation level and protein expression were found both in somatosensory cortex and hippocampus after ischemia preconditioning(P>0.05,n=6).Conclusion The development of cerebral ischemia preconditioning of rat might be not involved the phosphorylation and protein expression of p38 MAPK.
