Effect of Erythropoietin on Inflammation of Cerebral Ischemia-reperfusion Injury in Rats
- VernacularTitle:促红细胞生成素对大鼠脑缺血再灌注损伤后炎症反应的影响
- Author:
Yongjun CHEN
;
Yonghong TANG
;
Hui LONG
- Publication Type:Journal Article
- Keywords:
erythropoietin, cerebral ischemia-repefusion, inflammatory, interleukin-1β, myeloperoxidase
- From:
Chinese Journal of Rehabilitation Theory and Practice
2008;14(8):729-730
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effect of erythropoietin(EPO)on inflammatory injury induced by focal cerebral ischemia/reperfusion in rats.Methods 54 male Sprague-Dawley rats were randomly divided into 3 groups:sham operation group,normal saline control group and EPO group.The focal cerebral ischemia-reperfusion injury model was made by suture-occluded method.The content of interleukin-1 β(IL-1β)and the activity of myeloperoxidease(MPO)were measured by radioimmunoassay and chromatoptometry at 6 h,24 h and 48 h reperfusion following ischemia 2 h.The scores of behavior obstacle in rats were assessed at 24 h.Results Compared with the normal saline control group,EPO group got better score of behavior obstacle(all P<0.05).The content of IL-1β and the activity of MPO in brain in the EPO group were significantly lower than those in the normal saline control group(all P<0.01).Compared with two reperfusion groups,the sham operation group had no obvious abnormal change in each measurement item.Conclusion EPO can reduce the content of IL-1β and inhibit the infiltration of leukoeyte,which can provide protective effect on cerebral ischemic-repefusion injury in rats.
