Maackiain inhibits proliferation and promotes apoptosis of nasopharyngeal carcinoma cells by inhibiting the MAPK/Ras signaling pathway.
10.1016/S1875-5364(23)60420-0
- Author:
Xing JIANG
1
,
2
,
3
;
Xiaonan YANG
4
;
Yanxia SHI
1
,
5
;
Yan LONG
1
,
5
;
Wenqing SU
6
,
7
;
Wendong HE
6
,
8
;
Kunhua WEI
9
;
Jianhua MIAO
1
,
10
Author Information
1. College of Pharmacy, Guangxi Medical University, Nanning 530021, China
2. Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement/Guangxi Engineering Research Center of TCM Resource Intelligent Creation, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China
3. The Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang 421001, China.
4. Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement/Guangxi Engineering Research Center of TCM Resource Intelligent Creation, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China. Electronic address: nannangood33@163.com.
5. Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement/Guangxi Engineering Research Center of TCM Resource Intelligent Creation, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China.
6. Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement/Guangxi Engineering Research Center of TCM Resource Intelligent Creation, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China
7. College of Pharmacy, Youjiang Medical University for Nationalities, Baise 533000, China.
8. Department of Pharmacy, Guangxi Medical University Affiliated Tumor Hospital, Nanning 530021, China.
9. Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement/Guangxi Engineering Research Center of TCM Resource Intelligent Creation, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China.
10. Guangxi Key Laboratory of Medicinal Resources Protection and Genetic Improvement/Guangxi Engineering Research Center of TCM Resource Intelligent Creation, Guangxi Botanical Garden of Medicinal Plants, Nanning 530023, China. Electronic address: mjh1962@vip.163.com.
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Cell cycle;
MAPK/Ras signaling pathway;
Maackiain;
Nasopharyngeal carcinoma;
Proliferation;
Transcriptome sequencing
- MeSH:
Humans;
Nasopharyngeal Carcinoma/pathology*;
Cell Line, Tumor;
Cell Proliferation;
Apoptosis;
Signal Transduction;
Nasopharyngeal Neoplasms/pathology*
- From:
Chinese Journal of Natural Medicines (English Ed.)
2023;21(3):185-196
- CountryChina
- Language:English
-
Abstract:
Nasopharyngeal carcinoma (NPC) is the third most common malignancy with a high recurrence and metastasis rate in South China. Natural compounds extracted from traditional Chinese herbal medicines have been developed and utilized for the treatment of a variety of cancers with modest properties and slight side effects. Maackiain (MA) is a type of flavonoid that was first isolated from leguminous plants, and it has been reported to relieve various nervous system disorders and exert anti-allergic as well as anti-inflammatory effects. In this study, we demonstrated that MA inhibited proliferation, arrested cell cycle and induced apoptosis in nasopharyngeal carcinoma CNE1 and CNE2 cells in vitro and in vivo. The expression of the related proteins associated with these processes were consistent with the above effects. Moreover, transcriptome sequencing and subsequent Western blot experiments revealed that inhibition of the MAPK/Ras pathway may be responsible to the anti-tumor effect of MA on NPC cells. Therefore, the effects of MA and an activator of this pathway, tertiary butylhydroquinone (TBHQ), alone or combination, were investigated. The results showed TBHQ neutralized the inhibitory effects of MA. These data suggest that MA exerts its anti-tumor effect by inhibiting the MAPK/Ras signaling pathway and it has the potential to become a treatment for patients with NPC.
