Exosomes derived from Nr-CWS pretreated MSCs facilitate diabetic wound healing by promoting angiogenesis via the circIARS1/miR-4782-5p/VEGFA axis.
10.1016/S1875-5364(23)60419-4
- Author:
Qiang LI
1
;
Lei GUO
1
;
Jian WANG
1
;
Shengjun TAO
1
;
Peisheng JIN
2
Author Information
1. Department of Plastic Surgery, the Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, China.
2. Department of Plastic Surgery, the Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, China. Electronic address: 100000401006@xzhmu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Angiogenesis;
Exosomes;
Mesenchymal stromal cells;
Nocardia rubra cell wall skeleton (Nr-CWS);
circIARS1
- MeSH:
Humans;
Endothelial Cells/metabolism*;
Exosomes/metabolism*;
Cell Wall Skeleton/metabolism*;
Neovascularization, Physiologic;
Wound Healing/physiology*;
MicroRNAs/metabolism*;
Diabetes Mellitus;
Vascular Endothelial Growth Factor A/metabolism*
- From:
Chinese Journal of Natural Medicines (English Ed.)
2023;21(3):172-184
- CountryChina
- Language:English
-
Abstract:
Mesenchymal stem cell (MSC)-derived exosomes (Exos) were reported to a prospective candidate in accelerating diabetic wound healing due to their pro-angiogenic effect. MSCs pretreated with chemistry or biology factors were reported to advance the biological activities of MSC-derived exosomes. Hence, this study was designed to explore whether exosomes derived from human umbilical cord MSCs (hucMSCs) preconditioned with Nocardia rubra cell wall skeleton (Nr-CWS) exhibited superior proangiogenic effect on diabetic wound repair and its underlying molecular mechanisms. The results showed that Nr-CWS-Exos facilitated the proliferation, migration and tube formation of endothelial cells in vitro. In vivo, Nr-CWS-Exos exerted great effect on advancing wound healing by facilitating the angiogenesis of wound tissues compared with Exos. Furthermore, the expression of circIARS1 increased after HUVECs were treated with Nr-CWS-Exos. CircIARS1 promoted the pro-angiogenic effects of Nr-CWS-Exos on endothelial cellsvia the miR-4782-5p/VEGFA axis. Taken together, those data reveal that exosomes derived from Nr-CWS-pretreated MSCs might serve as an underlying strategy for diabetic wound treatment through advancing the biological function of endothelial cells via the circIARS1/miR-4782-5p/VEGFA axis.
