Screening the effective components in treating dampness stagnancy due to spleen deficiency syndrome and elucidating the potential mechanism of Poria water extract.
10.1016/S1875-5364(23)60392-9
- Author:
Huijun LI
1
;
Dandan ZHANG
1
;
Tianhe WANG
1
;
Xinyao LUO
1
;
Heyuan XIA
1
;
Xiang PAN
1
;
Sijie HAN
1
;
Pengtao YOU
1
;
Qiong WEI
1
;
Dan LIU
1
;
Zhongmei ZOU
2
,
3
;
Xiaochuan YE
4
Author Information
1. Hubei Key Laboratory of Resources and Chemistry of Chinese Medicine, School of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China.
2. Hubei Key Laboratory of Resources and Chemistry of Chinese Medicine, School of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China
3. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China. Electronic address: zmzou@implad.ac.cn.
4. Hubei Key Laboratory of Resources and Chemistry of Chinese Medicine, School of Pharmacy, Hubei University of Chinese Medicine, Wuhan 430065, China. Electronic address: Chuan9069@hbtcm.edu.cn.
- Publication Type:Journal Article
- Keywords:
Dampness stagnancy due to spleen deficiency syndrome;
Effective components;
Mechanisms;
Poria water extract;
Spectrum-effect relationship
- MeSH:
Animals;
Rats;
Poria;
Spleen;
Albumins;
Chromatography, Liquid;
Cyclic AMP Response Element-Binding Protein
- From:
Chinese Journal of Natural Medicines (English Ed.)
2023;21(2):83-98
- CountryChina
- Language:English
-
Abstract:
Poria is an important medicine for inducing diuresis to drain dampness from the middle energizer. However, the specific effective components and the potential mechanism of Poria remain largely unknown. To identify the effective components and the mechanism of Poria water extract (PWE) to treat dampness stagnancy due to spleen deficiency syndrome (DSSD), a rat model of DSSD was established through weight-loaded forced swimming, intragastric ice-water stimulation, humid living environment, and alternate-day fasting for 21 days. After 14 days of treatment with PWE, the results indicated that PWE increased fecal moisture percentage, urine output, D-xylose level and weight; amylase, albumin, and total protein levels; and the swimming time of rats with DSSD to different extents. Eleven highly related components were screened out using the spectrum-effect relationship and LC-MS. Mechanistic studies revealed that PWE significantly increased the expression of serum motilin (MTL), gastrin (GAS), ADCY5/6, p-PKAα/β/γ cat, and phosphorylated cAMP-response element binding protein in the stomach, and AQP3 expression in the colon. Moreover, it decreased the levels of serum ADH, the expression of AQP3 and AQP4 in the stomach, AQP1 and AQP3 in the duodenum, and AQP4 in the colon. PWE induced diuresis to drain dampness in rats with DSSD. Eleven main effective components were identified in PWE. They exerted therapeutic effect by regulating the AC-cAMP-AQP signaling pathway in the stomach, MTL and GAS levels in the serum, AQP1 and AQP3 expression in the duodenum, and AQP3 and AQP4 expression in the colon.
