Ferulic acid enhances insulin secretion by potentiating L-type Ca2+ channel activation.
10.1016/j.joim.2022.11.003
- Author:
Katesirin RUAMYOD
1
;
Wattana B WATANAPA
2
;
Chanrit KAKHAI
1
;
Pimchanok NAMBUNDIT
1
;
Sukrit TREEWAREE
1
;
Parin WONGSANUPA
1
Author Information
1. Department of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
2. Department of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. Electronic address: wattana.wat@mahidol.ac.th.
- Publication Type:Research Support, Non-U.S. Gov't
- Keywords:
Calcium channels, L-type;
Diabetes mellitus, type 2;
Ferulic acid;
Insulin;
Insulin-secreting cells;
Patch-clamp techniques
- MeSH:
Rats;
Animals;
Insulin Secretion;
Insulin/pharmacology*;
Insulin-Secreting Cells/metabolism*;
Coumaric Acids/metabolism*;
Calcium/metabolism*
- From:
Journal of Integrative Medicine
2023;21(1):99-105
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate the effect of ferulic acid, a natural compound, on pancreatic beta cell viability, Ca2+ channels, and insulin secretion.
METHODS:We studied the effects of ferulic acid on rat insulinoma cell line viability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide viability assay. The whole-cell patch-clamp technique and enzyme-linked immunosorbent assay were also used to examine the action of ferulic acid on Ca2+ channels and insulin secretion, respectively.
RESULTS:Ferulic acid did not affect cell viability during exposures up to 72 h. The electrophysiological study demonstrated that ferulic acid rapidly and concentration-dependently increased L-type Ca2+ channel current, shifting its activation curve in the hyperpolarizing direction with a decreased slope factor, while the voltage dependence of inactivation was not affected. On the other hand, ferulic acid have no effect on T-type Ca2+ channels. Furthermore, ferulic acid significantly increased insulin secretion, an effect inhibited by nifedipine and Ca2+-free extracellular fluid, confirming that ferulic acid-induced insulin secretion in these cells was mediated by augmenting Ca2+ influx through L-type Ca2+ channel. Our data also suggest that this may be a direct, nongenomic action.
CONCLUSION:This is the first electrophysiological demonstration that acute ferulic acid treatment could increase L-type Ca2+ channel current in pancreatic β cells by enhancing its voltage dependence of activation, leading to insulin secretion.
