Mutation-associated transcripts reconstruct the prognostic features of oral tongue squamous cell carcinoma.
10.1038/s41368-022-00210-3
- Author:
Libo LIANG
1
;
Yi LI
2
;
Binwu YING
3
;
Xinyan HUANG
4
;
Shenling LIAO
3
;
Jiajin YANG
4
;
Ga LIAO
5
Author Information
1. General Practice Medical Center, West China Hospital, Sichuan University, Chengdu, China.
2. State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
3. Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu, China.
4. West China School/Hospital of Stomatology, Sichuan University, Chengdu, China.
5. State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China. liaoga@hotmail.com.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Humans;
Biomarkers, Tumor;
Nerve Tissue Proteins;
Prognosis;
Squamous Cell Carcinoma of Head and Neck/pathology*;
Tongue Neoplasms/pathology*
- From:
International Journal of Oral Science
2023;15(1):1-1
- CountryChina
- Language:English
-
Abstract:
Tongue squamous cell carcinoma is highly malignant and has a poor prognosis. In this study, we aimed to combine whole-genome sequencing, whole-genome methylation, and whole-transcriptome analyses to understand the molecular mechanisms of tongue squamous cell carcinoma better. Oral tongue squamous cell carcinoma and adjacent normal tissues from five patients with tongue squamous cell carcinoma were included as five paired samples. After multi-omics sequencing, differentially methylated intervals, methylated loop sites, methylated promoters, and transcripts were screened for variation in all paired samples. Correlations were analyzed to determine biological processes in tongue squamous cell carcinoma. We found five mutated methylation promoters that were significantly associated with mRNA and lncRNA expression levels. Functional annotation of these transcripts revealed their involvement in triggering the mitogen-activated protein kinase cascade, which is associated with cancer progression and the development of drug resistance during treatment. The prognostic signature models constructed based on WDR81 and HNRNPH1 and combined clinical phenotype-gene prognostic signature models showed high predictive efficacy and can be applied to predict patient prognostic risk in clinical settings. We identified biological processes in tongue squamous cell carcinoma that are initiated by mutations in the methylation promoter and are associated with the expression levels of specific mRNAs and lncRNAs. Collectively, changes in transcript levels affect the prognosis of tongue squamous cell carcinoma patients.
