Intermittent heat exposure induces thoracic aorta injury in spontaneously hypertensive rats by activating the AMPK/mTOR/ULK1 pathway.
10.12122/j.issn.1673-4254.2023.02.05
- Author:
Chun Li YANG
1
;
Shu Jing XUE
2
;
Xiao Min WU
2
;
Ling HOU
2
;
Tao XU
2
;
Guang Hua LI
3
Author Information
1. Department of Nursing, General Hospital of Ningxia Medical University, Ningxia Medical University, Yinchuan 750004, China.
2. Department of Physiology, Ningxia Medical University, Yinchuan 750004, China.
3. School of Public Health and Management, Ningxia Medical University, Yinchuan 750004, China.
- Publication Type:Journal Article
- Keywords:
apoptosis;
autophagy;
heat exposure;
spontaneously hypertensive rat
- MeSH:
Rats;
Male;
Animals;
Rats, Inbred SHR;
AMP-Activated Protein Kinases/metabolism*;
bcl-2-Associated X Protein/metabolism*;
Aorta, Thoracic;
Beclin-1;
Hot Temperature;
TOR Serine-Threonine Kinases/metabolism*;
Proto-Oncogene Proteins c-bcl-2/metabolism*;
Apoptosis;
Aortic Diseases;
Autophagy;
Autophagy-Related Protein-1 Homolog/metabolism*
- From:
Journal of Southern Medical University
2023;43(2):191-198
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of different manners of heat exposure on thoracic aorta injury in spontaneously hypertensive rats (SHRs) and explore the underlying mechanism.
METHODS:Normal 6 to 7-week-old male SHRs were randomized into control group (cage at room temperature), intermittent heat exposure group (SHR-8 group, exposed to 32 ℃ for 8 h daily for 7 days) and SHR-24 group (with continuous exposure to 32 ℃ for 7 days). After the treatments, the pathologies of the thoracic aorta of the rats were observed with HE staining, and the expressions of Beclin1, LC3B and p62 were detected with Western blotting and immunofluorescence assay; TUNEL staining was used to observe cell apoptosis in the thoracic aorta, and the expressions of caspase-3, Bax, and Bcl-2 were detected using Western blotting. The effects of intraperitoneal injections of 3-MA (an autophagy agonist), rapamycin (an autophagy inhibitor) or compound C 30 min before intermittent heat exposure on the expressions of proteins associated with autophagy, apoptosis and the AMPK/mTOR/ULK1 pathway in the aorta were examined with immunohistochemistry.
RESULTS:In SHR-8 group, the rats showed incomplete aortic intima with disordered cell distribution and significantly increased expressions of Beclin1, LC3II/LC3I and Bax, lowered expressions of p62 and Bcl-2, and increased apoptotic cells in the thoracic aorta (P < 0.05). Pretreatment with 3-MA obviously inhibited the expressions of autophagy- and apoptosis-related proteins, whereas rapamycin promoted their expressions. Compared with the control group, the rats in SHR-8 group had significantly down-regulated p-mTOR and up-regulated p-AMPK and p-ULK1 expression of in the aorta; Treatment with compound C obviously lowered the expressions of p-AMPK and p-ULK1 and those of LC3B and Beclin1 as well.
CONCLUSION:In SHRs, intermittent heat exposure causes significant pathologies and promotes autophagy and apoptosis in the thoracic aorta possibly by activating the AMPK/mTOR/ULK1 pathway.
