Licochalcone A induces cell cycle arrest in human lung squamous carcinoma cells via the PI3K/Akt signaling pathway.
10.12122/j.issn.1673-4254.2023.01.15
- Author:
Xiao Li FAN
1
;
Juan WANG
1
;
Li Ming WANG
2
Author Information
1. College of Pharmacy, Guilin Medical University, Guilin 541100, China.
2. College of Clinical Medicine, Guilin Medical University, Guilin 541001, China.
- Publication Type:Journal Article
- Keywords:
PI3K/Akt signaling pathway;
cell cycle;
cell proliferation;
licochalcone A
- MeSH:
Humans;
Animals;
Mice;
Cyclin D1;
Phosphatidylinositol 3-Kinases;
Proto-Oncogene Proteins c-akt;
Carcinoma, Non-Small-Cell Lung;
Carcinoma, Squamous Cell;
Cell Cycle Checkpoints;
Lung Neoplasms;
Signal Transduction;
Lung
- From:
Journal of Southern Medical University
2023;43(1):111-116
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of licochalcone A (LCA) on the proliferation and cell cycle of human lung squamous carcinoma cells and explore its possible molecular mechanism.
METHODS:MTT assay was used to detect the changes in proliferation of H226 cells after treatment with different concentrations of LCA for 48 h, and the IC50 of LCA was calculated. Flow cytometry was used to analyze cell cycle changes in H226 cells treated with 10, 20, and 40 μmol/L LCA, and the expressions of cyclin D1, cyclin-dependent kinase CDK2 and CDK4, and p-PI3K, PI3K, p-Akt, and Akt in the treated cells were detected using Western blotting. The effect of intraperitoneal injection of LCA for 24 days on tumor volume and weight was assessed in a BALB/c-nu mouse model bearing lung squamous carcinoma xenografts.
RESULTS:MTT assay showed that LCA significantly decreased the viability of H226 cells with an IC50 of 28.3 μmol/L at 48 h. Flow cytometry suggested that LCA treatment induced obvious cell cycle arrest at the G1 phase. LCA treatment also significantly decreased the expressions of cyclin D1, CDK2, and CDK4, and inhibited the phosphorylation of PI3K and Akt in H226 cells. In the tumor-bearing mice, LCA treatment for 24 days significantly reduced the tumor volume and weight.
CONCLUSION:LCA is capable of inhibiting the proliferation and inducing cell cycle arrest in lung squamous carcinoma cells possibility by regulating the PI3K/Akt singling pathway.
