TRPV1 participates in the protective effect of propolis on colonic tissue of ulcerative colitis.
10.11817/j.issn.1672-7347.2023.220426
- Author:
Jing WANG
1
;
Zhen QIAN
2
;
Taiyu LU
2
;
Ruirui LI
2
;
Hui LI
3
;
Hao ZHANG
2
;
Li SUN
4
;
Haihua WANG
5
Author Information
1. Department of Physiology, School of Basic Medical Sciences, Wannan Medical College, Wuhu Anhui 241002. 247948083@qq.com.
2. Clinical Medical School, Wannan Medical College, Wuhu Anhui 241002.
3. Graduate School, Wannan Medical College, Wuhu Anhui 241002, China.
4. Department of Physiology, School of Basic Medical Sciences, Wannan Medical College, Wuhu Anhui 241002.
5. Department of Physiology, School of Basic Medical Sciences, Wannan Medical College, Wuhu Anhui 241002. 840099827@qq.com.
- Publication Type:Journal Article
- Keywords:
inflammatory medium;
propolis;
transient receptor potential vanilloid 1;
ulcerative colitis
- MeSH:
Animals;
Male;
Rats;
Antineoplastic Agents/therapeutic use*;
Colitis, Ulcerative/chemically induced*;
Colon/pathology*;
Disease Models, Animal;
Interleukin-6/pharmacology*;
Propolis/therapeutic use*;
Rats, Sprague-Dawley;
Sulfasalazine/therapeutic use*;
TRPV Cation Channels;
Tumor Necrosis Factor-alpha/pharmacology*
- From:
Journal of Central South University(Medical Sciences)
2023;48(2):182-190
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:Ulcerative colitis (UC) is a type of inflammatory bowel disease (IBD) mainly characterized by inflammation, ulceration and erosion of colonic mucosa and submucosa. Transient receptor potential vanilloid 1 (TRPV1) is an important mediator of visceral pain and inflammatory bowel disease. This study aims to investigate the protective effect of water soluble propolis (WSP) on UC colon inflammatory tissue and the role of TRPV1.
METHODS:Male SD rats were randomly divided into 6 groups (n=8): a normal control (NC) group, an ulcerative colitis model (UC) group, a low-WSP (L-WSP) group, a medium-WSP (M-WSP) group, a high-WSP (H-WSP) group, and a salazosulfapyridine (SASP) group. The rats in the NC group drank water freely, and the other groups drank 4% dextran sulfate sodium (DSS) solution freely for 7 d to replicate the ulcerative colitis model. Based on the successful replication of the UC, the L-WSP, M-WSP, and H-WSP groups were given 50, 100, and 200 mg/kg of water-soluble propolis by gavage for 7 d, and the SASP group was given 100 mg/kg of sulfasalazine by gavage for 7 d. The body weight of rats in each group was measured at the same time every day, the fecal traits and occult blood were observed to record the disease activity index (DAI). After intragastric administration, the animals were sacrificed after fasted 24 h. Serum and colonic tissue were collected, and the changes of MDA, IL-6 and TNF-α were detected. The pathological changes of colon tissues were observed by HE staining, and the expression of TRPV1 in colon tissues was observed by Western blotting, immunohistochemistry, and immunofluorescence.
RESULTS:The animals in each group that drank DSS freely showed symptoms such as weight loss, decreased appetite, depressed state, and hematochezia, indicating that the model was successfully established. Compared with the NC group, DAI scores of other groups were increased (all P<0.05). MDA, IL-6, TNF-α in serum and colon tissues of the UC group were increased compared with the NC group (all P<0.01), and they were decreased after WSP and SASP treatment (all P<0.01). The results of showed that the colon tissue structure was obviously broken and inflammatory infiltration in the UC group, while the H-WSP group and the SASP group significantly improved the colon tissue and alleviated inflammatory infiltration. The expression of TRPV1 in colon tissues in the UC group was increased compared with the NC group (all P<0.01), and it was decreased after WSP and SASP treatment.
CONCLUSIONS:WSP can alleviate the inflammatory state of ulcerative colitis induced by DSS, which might be related to the inhibition of inflammatory factors release, and down-regulation or desensitization of TRPV1.
