Application of modified Matutes score containing CD200 in diagnosis of chronic lymphocytic leukemia.
10.11817/j.issn.1672-7347.2022.220302
- Author:
Wenjin LI
1
;
Peng FANG
2
;
Gan FU
2
;
Hui LIANG
2
;
Minyuan PENG
3
Author Information
1. Department of Hematology, Jiangxi Pingxiang People's Hospital, Pingxiang Jiangxi 337055. liwenjin11@sina.com.
2. Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008.
3. Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008. pengminyuan@aliyun.com.
- Publication Type:Journal Article
- Keywords:
CD200;
chronic lymphocytic leukemia;
flow cytometry;
immunophenotyping score system
- MeSH:
Humans;
Adult;
Leukemia, Lymphocytic, Chronic, B-Cell/pathology*;
Retrospective Studies;
B-Lymphocytes/pathology*;
Lymphoma, Mantle-Cell/pathology*;
Diagnosis, Differential;
Lymphoma, B-Cell, Marginal Zone;
Flow Cytometry/methods*
- From:
Journal of Central South University(Medical Sciences)
2022;47(12):1689-1694
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:Immunophenotyping technique is a powerful tool for the diagnosis and differential diagnosis of chronic lymphocytic leukemia (CLL) and other B-cell chronic lymphoproliferative diseases (B-CLPD). CD200 is strongly expressed in CLL. This study aims to analyze the clinical value of modified Matutes score (MMS) containing CD200 in the diagnosis of CLL.
METHODS:We retrospectively analyzed 103 B-CLPD patients diagnosed from January 2020 to July 2021, including 64 CLL patients, 11 follicular lymphoma (FL) patients, 14 mantle cell lymphoma (MCL) patients, 6 marginal zone lymphoma (MZL) patients, 1 hairy cell leukemia (HCL) patient, and 7 lymphoplasmic lymphoma/Waldenstrom macroglobulinemia (LPL/WM) patients. The expression of CD markers between the CLL group and the non-CLL group was compared, and the sensitivity, specificity, and clinical consistency of MMS and Royal Marsden Hospital (RMH) immunophenotyping score system were analyzed.
RESULTS:There were significant differences in the expressions of CD5, CD23, FMC7, CD22, CD79b, CD200, and sIg between the CLL group and the non-CLL group (χ2 values were 37.42, 54.98, 30.71, 11.67, 55.26, 68.48, and 17.88, respectively, all P<0.01). When the RMH immunophenotyping score≥4, the sensitivity was 79.7%, and the specificity was 100%. When the MMS≥3, the sensitivity was 95.3%, and the specificity was 100%. The Kappa coefficient of RMH immunophenotyping system was 0.677, and the Kappa coefficient of MMS system was 0.860.
CONCLUSIONS:The MMS system containing CD200 has better sensitivity and same specificity compared with RMH immunophenotyping system, and MMS system may be more useful in the diagnosis of CLL.
