Effects of miR-144-3p on Proliferation, Cell Cycle and Apoptosis of K562 Cells.
10.19746/j.cnki.issn.1009-2137.2023.01.012
- Author:
Ya KANG
1
;
Jin-Xia HAO
2
;
Su-Hua WEI
1
;
Meng-Chang WANG
3
Author Information
1. Department of Hematology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
2. Xi'an Jiaotong University Hospital, Xi'an 710061, Shaanxi Province, China.
3. Department of Hematology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.E-mail: swallow3956@sina.com.
- Publication Type:Journal Article
- Keywords:
K562 cells;
apoptosis;
cell cycle;
miR-144-3p;
proliferation
- MeSH:
Humans;
Apoptosis/genetics*;
Cell Cycle/genetics*;
Cell Line, Tumor;
Cell Proliferation/genetics*;
K562 Cells;
MicroRNAs/metabolism*
- From:
Journal of Experimental Hematology
2023;31(1):76-80
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of miR-144-3p on cell proliferation, cell cycle and apoptosis of blast phase chronic myelogenous leukemia (CML) K562 cells.
METHODS:K562 cells were cultured in vitro and mimics negative control, hsa-miR-144-3p mimics, inhibitor negative control and miR-144-3p inhibitor were respectively transfected into K562 cells with transfection reagents. The cells were divided into five groups including blank control, mimics negative control, miR-144-3p mimics, inhibitor negative control and miR-144-3p inhibitor. After transfection, the cell proliferation activity was detected by CCK-8 assay. The cell cycle distribution and apoptosis were detected by flow cytometry.
RESULTS:Compared with the blank control and mimics negative control groups, the proliferation rate of miR-144-3p mimics group was significantly decreased (P<0.05), the proportion of S phase cells was markedly increased (P<0.05), while the proportion of G1 phase cells was obviously decreased (P<0.05), and the apoptosis rate was significantly increased (P<0.05). Compared with the blank control and inhibitor negative control groups, the proliferation rate of miR-144-3p inhibitor group was obviously increased (P<0.05), the proportion of S phase cells was markedly decreased (P<0.05), while the proportion of G1 phase cells was obviously increased (P<0.05), and the apoptosis rate was significantly decreased (P<0.05).
CONCLUSION:miR-144-3p can inhibit the proliferation and promote apoptosis of K562 cells, affect the cell cycle, and block K562 cells in S phase, which indicates that miR-144-3p is involved in the cell cycle activity of CML during blastic phase.
