Effect of Dichloromethane Extraction Phase of Patrinia Scabiosaefolia Fisch. Stem on Proliferation and Differentiation of K562 Cells.
10.19746/j.cnki.issn.1009-2137.2023.01.004
- Author:
Le-Yuan MI
1
;
Ke-Jing LI
2
;
Shan LI
2
;
Ting LIU
2
;
Xiao-Jing CHAI
3
;
Ying ZHANG
3
;
Juan LI
4
Author Information
1. The First Clinical Medical College of Lanzhou University, Lanzhou 730000, Gansu Province, China,Clinical Laboratory Center of Gansu Provincial Maternity and Child-care Hospital, Lanzhou 730050, Gansu Province, China.
2. The First Clinical Medical College of Lanzhou University, Lanzhou 730000, Gansu Province, China.
3. Central Laboratory of The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China.
4. The First Clinical Medical College of Lanzhou University, Lanzhou 730000, Gansu Province, China,Central Laboratory of The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, China,Gansu Key Laboratory of Genetic Study of Hematopathy, Lanzhou 730000, Gansu Province, China,E-mail: lj67629@163.com.
- Publication Type:Journal Article
- Keywords:
K562 cell line;
Patrinia scabiosaefolia Fisch. Stem;
cell apoptosis;
dichloromethane extraction phase;
induced differentiation
- MeSH:
Humans;
K562 Cells;
Patrinia;
Methylene Chloride/pharmacology*;
Apoptosis;
Cell Proliferation;
Cell Differentiation
- From:
Journal of Experimental Hematology
2023;31(1):25-32
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of dichloromethane extraction phase of ethanol extract from stem of Patrinia scabiosaefolia Fisch.(DPSS) on proliferation and differentiation of K562 cells and its related mechanism.
METHODS:MTT assay was used to detect the effects of DPSS at 0, 25, 50, 100 and 200 μg/ml on the proliferation of K562 cells at 24, 48 and 72 hours. Flow cytometry was used to analyze the changes of cell cycle and apoptosis at 24 and 48 hours. Wright-Giemsa staining was used to observe the morphological changes of K562 cells. The cell surface antigens CD33 and CD11b were detected by flow cytometry.
RESULTS:The proliferation of K562 cells treated with different concentrations of DPSS was inhibited in a time-dose dependent manner (r=-0.96). Cell cycle analysis showed that with the increase of DPSS concentration, cells in G2/M phase increased (r=0.88), and cells were blocked in G2/M phase. Flow cytometry results showed that with the apoptosis rate of K562 cells was the highest when treated with 200 μg/ml DPSS for 48 h. Morphological observation showed that the K562 cell body increased, the amount of cytoplasm increased, the ratio of nucleus to cytoplasm decreased, and the nuclear chromatin was rough after DPSS treatment. Cell differentiation antigen, CD33 and CD11b, were positively expressed after treated with DPSS.
CONCLUSION:DPSS can induce apoptosis through cell cycle arrest, inhibit the proliferation of K562 cells, and induce K562 cells to differentiate into monocytes, which has a potential anti-leukemia effect.
