Influence of E-cadherin methylation on prognosis in children with acute lymphoblastic leukemia.
10.7499/j.issn.1008-8830.2208018
- Author:
Feng-Qi QI
1
;
Wei HAN
1
;
Jing YAN
1
;
Cong XIN
1
;
Yan LI
1
;
Lei GUO
1
;
Wen-Peng WANG
1
;
Ji-Zhao GAO
1
Author Information
1. Department of Pediatric Hematology and Oncology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu 221002, China.
- Publication Type:Journal Article
- Keywords:
Acute lymphoblastic leukemia;
Child;
E-cadherin;
Methylation;
Prognosis
- MeSH:
Child;
Humans;
Cadherins/genetics*;
DNA Methylation;
Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics*;
Prognosis;
RNA, Messenger
- From:
Chinese Journal of Contemporary Pediatrics
2023;25(1):46-50
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVES:To study the significance of E-cadherin and the association between E-cadherin methylation status and prognosis in children with acute lymphoblastic leukemia (ALL) by examining the mRNA and protein expression of E-cadherin and its gene methylation status in bone marrow mononuclear cells of children with ALL.
METHODS:The samples of 5 mL bone marrow blood were collected from 42 children with ALL who were diagnosed for the first time at diagnosis (pre-treatment group) and on day 33 of induction chemotherapy (post-treatment group). RT-qPCR, Western blot, and methylation-specific PCR were used to measure the mRNA and protein expression of E-cadherin and the methylation level of the E-cadherin gene. The changes in each index after induction chemotherapy were compared.
RESULTS:The mRNA and protein expression levels of E-cadherin in the post-treatment group were significantly higher than those in the pre-treatment group (P<0.05), while the positive rate of E-cadherin gene methylation in the post-treatment group was significantly lower than that in the pre-treatment group (P<0.05). At the end of the test, the children with negative methylation had significantly higher overall survival rate and event-free survival rate than those with positive methylation (P<0.05).
CONCLUSIONS:E-cadherin expression is associated with the development of ALL in children, and its decreased expression and increased methylation level may indicate a poor prognosis.
