Analysis of the effect of midazolam on pain in a rat model of lumbar disc herniation based on the p38 MAPK signaling pathway.
10.12200/j.issn.1003-0034.2023.01.010
- Author:
Jian LIU
1
;
Yu-Jun YE
2
;
Shu-Min LIU
1
;
Shuang LIU
3
Author Information
1. Department of Anesthesiology, Kailuan General Hospital Zhaogezhuang Hospital, Guzhi 063101, Hebei, China.
2. Department of Anesthesiology, Tangshan Hospital of Traditional Chinese Medicine, Tangshan 063000, Hebei, China.
3. Department of Surgery, Kailuan General Hospital Zhaogezhuang Hospital, Guzhi 063101, Hebei, China.
- Publication Type:Journal Article
- Keywords:
Immunity;
Inflammation;
Intervertebral disk displacement;
Lumbar vertebrae;
Pain;
p38MAPK signaling pathway
- MeSH:
Rats;
Male;
Female;
Animals;
Intervertebral Disc Displacement/pathology*;
Rats, Sprague-Dawley;
Matrix Metalloproteinase 3/metabolism*;
Midazolam;
Tumor Necrosis Factor-alpha/metabolism*;
Serotonin/metabolism*;
MAP Kinase Signaling System/physiology*;
Pain;
p38 Mitogen-Activated Protein Kinases/metabolism*
- From:
China Journal of Orthopaedics and Traumatology
2023;36(1):55-60
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of midazolam on pain in lumbar disc herniation model rats based on p38 MAPK signaling pathway.
METHODS:Fifty SPF-grade Sprague-Dawley healthy rats, half male and half female, were selected and randomly divided into normal group, model group, and low-dose, medium-dose, high-dose groups. Model group and low-dose, medium-dose, high-dose groups were initially modeled for lumbar disc herniation. Intraperitoneal injection of saline was performed in rats of normal and model groups; and in the low-dose, medium-dose, and high-dose groups, intraperitoneal injection of midazolam was performed with doses of 30, 60, and 90 mg/kg, respectively. Interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), 5-hydroxytryptamine (5-HT), β-endorphin (β-EP), substance P (SP), neuropeptide Y (NPY) were detected in the serum of rats by enzyme-linked immunoassay. The expression of p38 MAPK and matrix metalloproteinase-3(MMP-3) protein were detected by Western blot in the tissues of rats of each group.
RESULTS:The levels of TNF-α, IL-1β and β-EP were higher and the level of 5-HT was lower in the model group than in the normal group(P<0.05);the levels of TNF-α, IL-1β and β-EP were lower and the level of 5-HT was higher in the low-dose, medium-dose and high-dose groups than in the model group(P<0.05). The levels of SP and NPY increased in the model group compared with the normal group (P<0.05) and the levels of SP and NPY decreased in the low-dose, medium-dose and high-dose groups compared with the model group (P<0.05). The expression of p38 MAPK and MMP-3 increased in the model group compared with the normal group (P<0.05); the expression of p38 MAPK and MMP-3 decreased in the low-dose, medium-dose and high-dose compared with the model group(P<0.05).
CONCLUSION:Midazolam may ameliorate the immune inflammatory response in rats with a model of lumbar disc herniation, possibly regulated through the p38MAPK signaling pathway.
