The role of Nrf2 in the alteration of tight junction protein expression in choroid plexus epithelial cells created by lanthanum-activated MMP9.
10.3760/cma.j.cn121094-20220121-00039
- VernacularTitle:Nrf2在镧活化MMP9致脉络丛上皮细胞紧密连接蛋白表达改变中的作用
- Author:
Jing SUN
1
;
Xing Bo XU
2
;
Hong Yue SU
2
;
Li Cheng YAN
3
;
Yan Shu ZHANG
4
;
Li Jin ZHANG
3
Author Information
1. Public Health and Preventive Medicine, School of Public Health, North China University of Science and Technology, Tangshan 063210, China.
2. Preventive Medicine, School of Public Health, North China University of Science and Technology, Tangshan 063210, China.
3. Department of Health Toxicology, School of Public Health, North China University of Science and Technology, Tangshan 063210, China.
4. Laboratory Animal Center, North China University of Science and Technology, Tangshan 063210, China.
- Publication Type:Journal Article
- Keywords:
Cell tight junctions;
Lanthanum;
Lanthanum chloride;
Matrix metalloproteinase 9;
Nuclear factor erythroid 2-related factor 2;
Reactive oxygen species
- MeSH:
Rats;
Animals;
Matrix Metalloproteinase 9/metabolism*;
NF-E2-Related Factor 2/metabolism*;
Tight Junction Proteins/metabolism*;
Occludin/pharmacology*;
Choroid Plexus/metabolism*;
Reactive Oxygen Species/metabolism*;
Lanthanum/pharmacology*;
Epithelial Cells;
Zonula Occludens-1 Protein/metabolism*;
Phosphoproteins/pharmacology*
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2023;41(1):2-7
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of nuclear factor erythroid 2-related factor 2 (Nrf2) in the alteration of tight junction protein expression in choroid plexus epithelial cells created by lanthanum-activated matrix metalloproteinase 9 (MMP9) . Methods: In October 2020, immortalized rat choroid plexus epithelial cell line (Z310) cells were used as the blood-cerebrospinal fluid barrier in vitro, and were divided into control group and 0.125, 0.25, 0.5 mmol/L lanthanum chloride (LaCl(3)) treatment group. After treating Z310 cells with different concentrations of LaCl(3) for 24 hours, the morphological changes of Z310 cells were observed under inverted microscope, the protein expression levels of MMP9, occludin and zonula occludens-1 (ZO-1) were observed by cellular immunofluorescence method, and the protein expression levels of MMP9, tissue inhibitors of metalloproteinase1 (TIMP1) , occludin, ZO-1 and Nrf2 were detected by Western blotting. The level of reactive oxygen species (ROS) in cells was detected by flow cytometry. Results: Compared with the control group, Z310 cells in the LaCl(3) treatment group were smaller in size, with fewer intercellular junctions, and more dead cells and cell fragments. The expression level of MMP9 protein in cells treated with 0.25 and 0.5 mmol/L LaCl(3) was significantly higher than that in the control group (P<0.05) , and the expression level of TIMP1 and tight junction proteins occudin and ZO-1 was significantly lower than that in the control group (P<0.05) . Compared with the control group, the ROS production level in the 0.25, 0.5 mmol/L LaCl(3) treatment group was significantly increased (P<0.05) , and the Nrf2 protein expression level in the 0.125, 0.25, 0.5 mmol/L LaCl(3) treatment group was significantly decreased (P<0.05) . Conclusion: Lanthanum may increase the level of ROS in cells by down regulating the expression of Nrf2, thus activating MMP9 to reduce the expression level of intercellular tight junction proteins occludin and ZO-1.
