Frontiers and development in live-cell super-resolution fluorescence microscopy.
10.7507/1001-5515.202210060
- Author:
Yufei CHENG
1
;
Wei LI
2
;
Tingting JIN
3
;
Sisi WU
1
;
Longhao ZHANG
2
Author Information
1. Core Facilities, West China Hospital of Sichuan University, Chengdu 610041, P. R. China.
2. Department of Medical Discipline Construction, West China hospital of Sichuan University, Chengdu 610041, P. R. China.
3. Department of Health Policy and Management, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, P. R. China.
- Publication Type:Journal Article
- Keywords:
Live-cell;
Minimal photon fluxes;
Stimulated emission depletion microscopy;
Structured illumination microscopy;
Super-resolution fluorescence microscopy
- MeSH:
Artificial Intelligence;
Microscopy, Fluorescence;
Fluorescent Dyes;
Technology
- From:
Journal of Biomedical Engineering
2023;40(1):180-184
- CountryChina
- Language:Chinese
-
Abstract:
This paper reviews the research progress on live-cell super-resolution fluorescence microscopy, discusses the current research status and hotspots in this field, and summarizes the technological application of super-resolution fluorescence microscopy for live-cell imaging. To date, this field has gained progress in numerous aspects. Specifically, the structured illumination microscopy, stimulated emission depletion microscopy, and the recently introduced minimal photon fluxes microscopy are the current research hotspots. According to the current progress in this field, future development trend is likely to be largely driven by artificial intelligence as well as advances in fluorescent probes and relevant labelling methods.
