Effects of repetitive transcranial magnetic stimulation on neuronal excitability and ion channels in hindlimb unloading mice.
10.7507/1001-5515.202205008
- Author:
Wentao HOU
1
;
Rui FU
1
;
Mingqiang ZHU
1
;
Haijun ZHU
1
;
Chong DING
1
Author Information
1. Hebei Key Laboratory of Bioelectromagnetics and Neural Engineering, School of Health Sciences & Biomedical Engineering, Hebei University of Technology, Tianjin 300130, P. R. China.
- Publication Type:Journal Article
- Keywords:
Hindlimb unloading mouse;
Nerve excitability;
Repetitive transcranial magnetic stimulation;
Voltage-gated potassium channels;
Voltage-gated sodium channels
- MeSH:
Animals;
Mice;
Transcranial Magnetic Stimulation;
Hindlimb Suspension;
Neurons;
Cognitive Dysfunction;
Brain
- From:
Journal of Biomedical Engineering
2023;40(1):8-19
- CountryChina
- Language:Chinese
-
Abstract:
Weightlessness in the space environment affects astronauts' learning memory and cognitive function. Repetitive transcranial magnetic stimulation has been shown to be effective in improving cognitive dysfunction. In this study, we investigated the effects of repetitive transcranial magnetic stimulation on neural excitability and ion channels in simulated weightlessness mice from a neurophysiological perspective. Young C57 mice were divided into control, hindlimb unloading and magnetic stimulation groups. The mice in the hindlimb unloading and magnetic stimulation groups were treated with hindlimb unloading for 14 days to establish a simulated weightlessness model, while the mice in the magnetic stimulation group were subjected to 14 days of repetitive transcranial magnetic stimulation. Using isolated brain slice patch clamp experiments, the relevant indexes of action potential and the kinetic property changes of voltage-gated sodium and potassium channels were detected to analyze the excitability of neurons and their ion channel mechanisms. The results showed that the behavioral cognitive ability and neuronal excitability of the mice decreased significantly with hindlimb unloading. Repetitive transcranial magnetic stimulation could significantly improve the cognitive impairment and neuroelectrophysiological indexes of the hindlimb unloading mice. Repetitive transcranial magnetic stimulation may change the activation, inactivation and reactivation process of sodium and potassium ion channels by promoting sodium ion outflow and inhibiting potassium ion, and affect the dynamic characteristics of ion channels, so as to enhance the excitability of single neurons and improve the cognitive damage and spatial memory ability of hindlimb unloading mice.
