Isolation and quantification of a 17-epi-dammarane triterpenoid rhuslactone from roots of Rhus chinensis and its preventive effects on coronary heart disease and thrombosis in zebrafish.
10.19540/j.cnki.cjcmm.20221227.201
- Author:
Ling-Jie RUAN
1
;
Li-Na HUANG
1
;
Xin-Yi GAO
2
;
Chang-Jie LAI
2
;
Lin-Jing ZHANG
2
;
Yu-Fan WU
2
;
Mei SHA
2
;
Miao YE
1
Author Information
1. College of Pharmacy,Fujian University of Traditional Chinese Medicine Fuzhou 350122,China Centre of Biomedical Research & Development,Fujian University of Traditional Chinese Medicine Fuzhou 350122,China.
2. College of Pharmacy,Fujian University of Traditional Chinese Medicine Fuzhou 350122,China.
- Publication Type:Journal Article
- Keywords:
17-epi-dammarane triterpenoid;
C-17 stereochemistry;
Rhus chinensis;
coronary heart disease;
qcc-NMR;
rhuslactone;
thrombosis
- MeSH:
Animals;
Zebrafish;
Rhus/chemistry*;
Triterpenes/analysis*;
Coronary Disease;
Thrombosis
- From:
China Journal of Chinese Materia Medica
2023;48(6):1558-1567
- CountryChina
- Language:Chinese
-
Abstract:
Based on mass spectrometry(MS)-guided separation strategy, compound 1 was obtained from the roots of Rhus chinensis. By comprehensive analysis of high resolution-electrospray ionization-mass spectrometry(HR-ESI-MS), nuclear magnetic resonance(NMR) data, and quantum chemical calculation of NMR(qcc-NMR) parameters, compound 1 was elucidated as rhuslactone, a 17-epi-dammarane triterpenoid with a rare 17α-side chain. An HPLC-ELSD method for its quantification in R. chinensis was established and adopted for the quantification of rhuslactone in different batches of R. chinensis. Rhuslactone displayed a good linear relationship within the range of 0.021 3-1.07 μmol·mL~(-1 )(r=0.997 6), and the average recovery was 99.34% [relative standard deviation(RSD) 2.9%). Moreover, the results of the evaluation test of the preventive effects of rhusalctone on coronary heart disease(CHD) and thrombosis showed that rhuslactone(0.11 nmol·mL~(-1)) significantly alleviated heart enlargement and venous congestion and increased cardiac output(CO), blood flow velocity(BFV), and heart rate, thereby reducing thrombus formation in zebrafish with CHD. The effects of rhuslactone on CO and BFV were superior to that of digoxin(1.02 nmol·mL~(-1)), and its effect on improving heart rate was comparable to that of digoxin. This study provides experimental references for the isolation, identification, quality control, and application of rhuslactone from R. chinensis against CHD. It is worth mentioning that this study has discussed some omissions in the determination of the stereochemistry of C-17 in dammarane triterpenoids in the present coursebook Chemistry of Chinese Medicine and some research papers, that is, the compound may be 17-epi-dammarane triterpenoid. This paper has also proposed steps for the establishment of C-17 stereochemistry.
