Mechanism of famous classical formula Huaihua Powder in treatment of ulcerative colitis based on metabonomics.
10.19540/j.cnki.cjcmm.20221025.401
- Author:
Li-Ying HAN
1
;
Hao YU
1
;
Tian-Jiao LI
2
;
Shuai WANG
2
;
Yong-Rui BAO
2
;
Xian-Sheng MENG
2
Author Information
1. College of Traditional Chinese Medicine, Liaoning University of Traditional Chinese Medicine Dalian 116600, China.
2. College of Traditional Chinese Medicine, Liaoning University of Traditional Chinese Medicine Dalian 116600, China Liaoning Professional Innovation Technology Center for Multidimensional Analysis of Traditional Chinese Medicine, Liaoning Modern Traditional Chinese Medicine Research Engineering Laboratory Dalian 116600, China.
- Publication Type:Journal Article
- Keywords:
Huaihua Powder;
metabolic pathway;
potential biomarker;
ulcerative colitis
- MeSH:
Mice;
Animals;
Colitis, Ulcerative/metabolism*;
Powders;
Tumor Necrosis Factor-alpha/metabolism*;
Interleukin-6/metabolism*;
Metabolomics;
Colon;
Disease Models, Animal;
Biomarkers;
Dextran Sulfate/therapeutic use*
- From:
China Journal of Chinese Materia Medica
2023;48(5):1300-1309
- CountryChina
- Language:Chinese
-
Abstract:
Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry(UHPLC-Q-TOF-MS) was employed in this study to observe the effect of Huaihua Powder on the serum metabolites of mice with ulcerative colitis and reveal the mechanism of Huaihua Powder in the treatment of ulcerative colitis. The mouse model of ulcerative colitis was established by dextran sodium sulfate salt(DSS). The therapeutic effect of Huaihua Powder on ulcerative colitis was preliminarily evaluated based on the disease activity index(DAI), colon appearance, colon tissue morphology, and the content of inflammatory cytokines such as tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), and interleukin-1β(IL-1β). UHPLC-Q-TOF-MS was employed to profile the endogenous metabolites of serum samples in blank control group, model group, and low-, medium-, and high-dose Huaihua Powder groups. Multivariate analyses such as principal component analysis(PCA), partial least squares discriminant analysis(PLS-DA), and orthogonal partial least squares discriminant analysis(OPLS-DA) were performed for pattern recognition. Potential biomarkers were screened by Mass Profiler Professional(MPP) B.14.00 with the thresholds of fold change≥2 and P<0.05. The metabolic pathways were enriched by MetaboAnalyst 5.0. The results showed that Huaihua Powder significantly improved the general state and colon tissue morphology of mice with ulcerative colitis, reduced DAI, and lowered the levels of TNF-α, IL-6, and IL-1β in serum. A total of 38 potential biomarkers were predicted to be related to the regulatory effect of Huaihua Powder, which were mainly involved in glycerophospholipid metabolism, glycine, serine, and threonine metabolism, mutual transformation of glucuronic acid, and glutathione metabolism. This study employed metabolomics to analyze the mechanism of Huaihua Powder in the treatment of ulcerative colitis, laying a foundation for the further research.
