Systematic comparison of two kinds of Bufonis Venenum derived from different Bufo gargarizans subspecies based on metabolomics and antitumor activity.
10.19540/j.cnki.cjcmm.20220627.202
- Author:
Bo SUN
1
;
Ming-Li LI
1
;
Yao-Hua DING
2
;
Yong ZHANG
3
;
Bo XIA
3
;
Sheng-Ya GUO
3
;
Shi-Lu WANG
2
;
Bao-Lin BIAN
1
;
Nan SI
1
;
Hai-Yu ZHAO
4
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
2. Xichang Fuhua Jinchan Biotechnology Co., Ltd. Xichang 615000, China.
3. Hunter Biotechnology Co., Ltd.,Hangzhou 310051, China.
4. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China Medicinal Toad Ecological Breeding Engineering Technology Research Center of National Forestry and Grassland Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Bufo gararizans andrewsi;
Bufo gargarizans gargarizans;
Bufonis Venenum;
active evaluation;
quality markers
- MeSH:
Animals;
Zebrafish;
Bufanolides/analysis*;
Bufonidae;
Chromatography, High Pressure Liquid;
Quality Control;
Cell Line, Tumor
- From:
China Journal of Chinese Materia Medica
2023;48(5):1280-1288
- CountryChina
- Language:Chinese
-
Abstract:
This paper compared the differences between two kinds of Bufonis Venenum produced by Bufo gargarizans gargarizans and B. gararizans andrewsi, and verified the rationality of the market value orientation of Bufonis Venenum based on the zebrafish mo-del. Twenty batches of Bufonis Venenum from Jiangsu province, Hebei province, Liaoning province, Jilin province, and Liangshan, Sichuan province, including B. gargarizans gargarizans and B. gararizans andrewsi, were collected. The UHPLC-LTQ-Orbitrap-MS combined with principal component analysis was used to compare the differences between two kinds of Bufonis Venenum. According to the limiting conditions of VIP>1, FC<0.5 or FC>2.0, and peak total area ratio>1%, 9 differential markers were determined, which were cinobufagin, cinobufotalin, arenobufagin, resibufogenin, scillaredin A, resibufagin, 3-(N-suberoylargininyl)-arenobufagin, 3-(N-suberoylargininyl)-marinobufagin, and 3-(N-suberoylargininyl)-resibufogenin. The content of 20 batches of Bufonis Venenum was determined according to the Chinese Pharmacopoeia(2020 edition) by high-performance liquid chromatography, and the 2 batches of Bufonis Venenum, CS7(8.99% of total content) and CS9(5.03% of total content), with the largest difference in the total content of the three quality control indexes of the Chinese Pharmacopoeia(bufalin, cinobufagin, and resibufogenin) were selected to evaluate their anti-liver tumor activity based on the zebrafish model. The tumor inhibition rates of the 2 batches were 38.06% and 45.29%, respectively, proving that only using the quality control indexes of the Chinese Pharmacopoeia as the value orientation of Bufonis Venenum market circulation was unreasonable. This research provides data support for the effective utilization of Bufonis Venenum resources and the establishment of a rational quality evaluation system of Bufonis Venenum.
