Main components from cultivated and wild Nardostachyos Radix et Rhizoma by LC-MS and GC-MS.
10.19540/j.cnki.cjcmm.20221210.101
- Author:
Ying LI
1
;
Hai-Rong ZHONG
2
;
Ri-Luo FENG
1
;
Tao WANG
1
;
Jiang LUO
1
;
Zheng-Ming YANG
3
;
Chen CHEN
3
;
Xin-Jia YAN
3
;
Xiao-Ming BAO
4
;
Wen-Bing LI
3
;
Yuan LIU
5
Author Information
1. College of Pharmacy, Southwest Minzu University Chengdu 610225, China Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of China, Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology and Engineering Laboratory Chengdu 610225, China.
2. Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of China, Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology and Engineering Laboratory Chengdu 610225, China Sichuan College of Traditional Chinese Medicine Mianyang 621000, China.
3. Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of China, Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology and Engineering Laboratory Chengdu 610225, China Institute of Qinghai-Tibetan Plateau, Southwest Minzu University Chengdu 610225, China.
4. SHIMADZU Enterprise Management (China) Co., Ltd. Chengdu 611900, China.
5. Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of China, Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology and Engineering Laboratory Chengdu 610225, China Ethnic Medicine Institute, Southwest Minzu University Chengdu 610225, China.
- Publication Type:Journal Article
- Keywords:
GC-MS;
Nardostachyos Radix et Rhizoma;
UPLC-Q-TOF-MS;
imitation wild cultivation;
quality evaluation
- MeSH:
Gas Chromatography-Mass Spectrometry;
Chromatography, Liquid;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/chemistry*;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2023;48(5):1218-1228
- CountryChina
- Language:Chinese
-
Abstract:
In this study, ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry(UPLC-Q-TOF-MS) and gas chromatography-mass spectrometry(GC-MS) were combined with non-targeted metabonomic analysis based on multivariate statistics analysis, and the content of five indicative components in nardosinone was determined and compared by UPLC. The main chemical components of Nardostachyos Radix et Rhizoma with imitative wild cultivation and wild Nardostachyos Radix et Rhizoma were comprehensively analyzed. The results of multivariate statistical analysis based on liquid chromatography-mass spectrometry(LC-MS) and GC-MS were consistent. G1 and G2 of the imitative wild cultivation group and G8-G19 of the wild group were clustered into category 1, while G7 of the wild group and G3-G6 of the imitative wild cultivation group were clustered into category 2. After removing the outlier data of G1, G2, and G7, G3-G6 of the imitative wild cultivation group were clustered into one category, and G8-G19 of the wild group were clustered into the other category. Twenty-six chemical components were identified according to the positive and negative ion modes detected by LC-MS. The content of five indicative components(VIP>1.5) was determined using UPLC, revealing that chlorogenic acid, isochlorogenic acid A, isochlorogenic acid C, linarin, nardosinone, and total content in the imitative wild cultivation group were 1.85, 1.52, 1.26, 0.90, 2.93, and 2.56 times those in the wild group, respectively. OPLS-DA based on GC-MS obtained 10 diffe-rential peaks. Among them, the relative content of α-humulene and aristolene in the imitative wild cultivation group were extremely significantly(P<0.01) and significantly(P<0.05) higher than that in the wild group, while the relative content of 7 components such as 5,6-epoxy-3-hydroxy-7-megastigmen-9-one, γ-eudesmol, and juniper camphor and 12-isopropyl-1,5,9-trimethyl-4,8,13-cyclotetrade-catriene-1,3-diol was extremely significantly(P<0.01) and significantly(P<0.05) lower than that in the wild group, respectively. Therefore, the main chemical components of the imitative wild cultivation group and wild group were basically the same. However, the content of non-volatile components in the imitative wild cultivation group was higher than that in the wild group, and the content of some volatile components was opposite. This study provides scientific data for the comprehensive evaluation of the quality of Nardostachyos Radix et Rhizoma with imitative wild cultivation and wild Nardostachyos Radix et Rhizoma.
