UPLC-Q-TOF-MS metabolomic study on improvement of acute myocardial ischemia in rats by Dalbergia cochinchinensis heartwood.
10.19540/j.cnki.cjcmm.20221024.401
- Author:
Wen-Long WANG
1
;
An LI
1
;
Lan-Ying CHEN
1
;
Jia-Rong LI
2
;
Ya-Ru CUI
3
;
Ni ZHANG
2
;
Ying-Ying LUO
1
;
Rong-Hua LIU
2
;
Can-Yue OUYANG
1
;
Bei-Xin YUAN
1
;
Ying ZHANG
1
;
Peng-Hao-Bang LIU
1
Author Information
1. National Pharmaceutical Engineering Centre for Solid Preparation of Chinese Herbal Medicine Nanchang 330006,China College of Pharmacy,Jiangxi University of Chinese Medicine Nanchang 330004,China.
2. College of Pharmacy,Jiangxi University of Chinese Medicine Nanchang 330004,China.
3. National Pharmaceutical Engineering Centre for Solid Preparation of Chinese Herbal Medicine Nanchang 330006,China.
- Publication Type:Journal Article
- Keywords:
Dalbergia cochinchinensis;
acute myocardial ischemia;
ligation of the left anterior descending coronary artery;
non-targeted metabolomics
- MeSH:
Male;
Animals;
Rats;
Rats, Sprague-Dawley;
Dalbergia;
Myocardial Ischemia;
Metabolomics;
Heart;
Heart Injuries;
Creatine Kinase, MB Form
- From:
China Journal of Chinese Materia Medica
2023;48(4):1043-1053
- CountryChina
- Language:Chinese
-
Abstract:
This paper aimed to study the effect of Dalbergia cochinchinensis heartwood on plasma endogenous metabolites in rats with ligation of the left anterior descending coronary artery, and to analyze the mechanism of D. cochinchinensis heartwood in improving acute myocardial ischemic injury. The stability and consistency of the components in the D. cochinchinensis heartwood were verified by the establishment of fingerprint, and 30 male SD rats were randomly divided into a sham group, a model group, and a D. cochinchinensis heartwood(6 g·kg~(-1)) group, with 10 rats in each group. The sham group only opened the chest without ligation, while the other groups established the model of ligation. Ten days after administration, the hearts were taken for hematoxylin-eosin(HE) staining, and the content of heart injury indexes in the plasma creatine kinase isoenzyme(CK-MB) and lactate dehydrogenase(LDH), energy metabolism-related index glucose(Glu) content, and vascular endothelial function index nitric oxide(NO) was determined. The endogenous metabolites were detected by ultra-high-performance liquid chromatography-time-of-flight-mass spectrometry(UPLC-Q-TOF-MS). The results showed that the D. cochinchinensis heartwood reduced the content of CK-MB and LDH in the plasma of rats to relieve myocardial injury, reduced the content of Glu in the plasma, improved myocardial energy metabolism, increased the content of NO, cured the vascular endothelial injury, and promoted vasodilation. D. cochinchinensis heartwood improved the increase of intercellular space, myocardial inflammatory cell infiltration, and myofilament rupture caused by ligation of the left anterior descending coronary artery. The metabolomic study showed that the content of 26 metabolites in the plasma of rats in the model group increased significantly, while the content of 27 metabolites decreased significantly. Twenty metabolites were significantly adjusted after the administration of D. cochinchinensis heartwood. D. cochinchinensis heartwood can significantly adjust the metabolic abnormality in rats with ligation of the left anterior descending coronary artery, and its mechanism may be related to the regulation of cardiac energy metabolism, NO production, and inflammation. The results provide a corresponding basis for further explaining the effect of D. cochinchinensis on the acute myocardial injury.
